Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)

Astilbin (5,7,3,4-tetrahydroxy-2,3-dihydroflavonol-3-ß-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra mollis, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and...

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Autores principales:	PETACCI,FERNANDO, FREITAS,SILVIA S, BRUNETTI,IGUATEMY L, KHALIL,NAJEH M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	Astilbin Myeloperoxidase Horseradish peroxidase Reactive Oxygen Species Hypochlorous acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100008
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spelling	oai:scielo:S0716-976020100001000082010-05-07Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)PETACCI,FERNANDOFREITAS,SILVIA SBRUNETTI,IGUATEMY LKHALIL,NAJEH M Astilbin Myeloperoxidase Horseradish peroxidase Reactive Oxygen Species Hypochlorous acid Astilbin (5,7,3,4-tetrahydroxy-2,3-dihydroflavonol-3-ß-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra mollis, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and horseradish peroxidase (HRP), and its antioxidant activity against hypochlorous acid (HOCl) and total antioxidant activity (TAC) by the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+). Astilbin inhibited MPO and HRP activities in a concentration-dependent relationship and effectively scavenged HOCl. The TAC by ABTS+ of astilbin (IC50 ~ 20 mM) was higher than that of uric acid, which was used as a positive control. These data demonstrate that astilbin is a potent antioxidant and that it inhibits MPO and HRP activities efficiently.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100008en10.4067/S0716-97602010000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Astilbin Myeloperoxidase Horseradish peroxidase Reactive Oxygen Species Hypochlorous acid
spellingShingle	Astilbin Myeloperoxidase Horseradish peroxidase Reactive Oxygen Species Hypochlorous acid PETACCI,FERNANDO FREITAS,SILVIA S BRUNETTI,IGUATEMY L KHALIL,NAJEH M Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
description	Astilbin (5,7,3,4-tetrahydroxy-2,3-dihydroflavonol-3-ß-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra mollis, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and horseradish peroxidase (HRP), and its antioxidant activity against hypochlorous acid (HOCl) and total antioxidant activity (TAC) by the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+). Astilbin inhibited MPO and HRP activities in a concentration-dependent relationship and effectively scavenged HOCl. The TAC by ABTS+ of astilbin (IC50 ~ 20 mM) was higher than that of uric acid, which was used as a positive control. These data demonstrate that astilbin is a potent antioxidant and that it inhibits MPO and HRP activities efficiently.
author	PETACCI,FERNANDO FREITAS,SILVIA S BRUNETTI,IGUATEMY L KHALIL,NAJEH M
author_facet	PETACCI,FERNANDO FREITAS,SILVIA S BRUNETTI,IGUATEMY L KHALIL,NAJEH M
author_sort	PETACCI,FERNANDO
title	Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
title_short	Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
title_full	Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
title_fullStr	Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
title_full_unstemmed	Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
title_sort	inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from dimorphandra mollis (fabaceae, caesalpinioideae)
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100008
work_keys_str_mv	AT petaccifernando inhibitionofperoxidaseactivityandscavengingofreactiveoxygenspeciesbyastilbinisolatedfromdimorphandramollisfabaceaecaesalpinioideae AT freitassilvias inhibitionofperoxidaseactivityandscavengingofreactiveoxygenspeciesbyastilbinisolatedfromdimorphandramollisfabaceaecaesalpinioideae AT brunettiiguatemyl inhibitionofperoxidaseactivityandscavengingofreactiveoxygenspeciesbyastilbinisolatedfromdimorphandramollisfabaceaecaesalpinioideae AT khalilnajehm inhibitionofperoxidaseactivityandscavengingofreactiveoxygenspeciesbyastilbinisolatedfromdimorphandramollisfabaceaecaesalpinioideae
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Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)

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