Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion

Normal bone remodeling is maintained by a balance between osteoclast and osteoblast activity, whereas defects in osteoclast activity affecting such balance result in metabolic bone disease. Macrophage-macrophage fusion leading to multinucleated osteoclasts being formed is still not well understood....

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Autores principales:	Murillo,Andrea, Guerrero,Carlos A, Acosta,Orlando, Cardozo,Carmen A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	bone resorption macrophage fusion osteoclast activity osteoclast differentiation PEG RANKL
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200009
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spelling	oai:scielo:S0716-976020100002000092010-09-24Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusionMurillo,AndreaGuerrero,Carlos AAcosta,OrlandoCardozo,Carmen A bone resorption macrophage fusion osteoclast activity osteoclast differentiation PEG RANKL Normal bone remodeling is maintained by a balance between osteoclast and osteoblast activity, whereas defects in osteoclast activity affecting such balance result in metabolic bone disease. Macrophage-macrophage fusion leading to multinucleated osteoclasts being formed is still not well understood. Here we present PEG-induced fusion of macrophages from both U937/A and J774 cell lines and the induced differentiation and activation of osteoclast-like cells according to the expression of osteoclast markers such as tartrate resistant acid phosphatase (TRAP) and bone resorptive activity. PEG-induced macrophage fusion, during the non-confuent stage, signifcantly increased the osteoclastogenic activity of macrophages from cell lines compared to that of spontaneous cell fusion in the absence of PEG (polyethylene glycol). The results shown in this work provide evidence that cell fusion per se induces osteoclast-like activity. PEG-fused macrophage differential response to pretreatment with osteoclastogenic factors was also examined in terms of its ability to form TRAP positive multinucleated cells (TPMNC) and its resorptive activity on bovine cortical bone slices. Our work has also led to a relatively simple method regarding those previously reported involving cell co-cultures. Multinucleated osteoclast-like cells obtained by PEG-induced fusion of macrophages from cell lines could represent a suitable system for conducting biochemical studies related to basic macrophage fusion mechanisms, bone-resorption activity and the experimental search for bone disease therapeutic alternatives.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.2 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200009en10.4067/S0716-97602010000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bone resorption macrophage fusion osteoclast activity osteoclast differentiation PEG RANKL
spellingShingle	bone resorption macrophage fusion osteoclast activity osteoclast differentiation PEG RANKL Murillo,Andrea Guerrero,Carlos A Acosta,Orlando Cardozo,Carmen A Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
description	Normal bone remodeling is maintained by a balance between osteoclast and osteoblast activity, whereas defects in osteoclast activity affecting such balance result in metabolic bone disease. Macrophage-macrophage fusion leading to multinucleated osteoclasts being formed is still not well understood. Here we present PEG-induced fusion of macrophages from both U937/A and J774 cell lines and the induced differentiation and activation of osteoclast-like cells according to the expression of osteoclast markers such as tartrate resistant acid phosphatase (TRAP) and bone resorptive activity. PEG-induced macrophage fusion, during the non-confuent stage, signifcantly increased the osteoclastogenic activity of macrophages from cell lines compared to that of spontaneous cell fusion in the absence of PEG (polyethylene glycol). The results shown in this work provide evidence that cell fusion per se induces osteoclast-like activity. PEG-fused macrophage differential response to pretreatment with osteoclastogenic factors was also examined in terms of its ability to form TRAP positive multinucleated cells (TPMNC) and its resorptive activity on bovine cortical bone slices. Our work has also led to a relatively simple method regarding those previously reported involving cell co-cultures. Multinucleated osteoclast-like cells obtained by PEG-induced fusion of macrophages from cell lines could represent a suitable system for conducting biochemical studies related to basic macrophage fusion mechanisms, bone-resorption activity and the experimental search for bone disease therapeutic alternatives.
author	Murillo,Andrea Guerrero,Carlos A Acosta,Orlando Cardozo,Carmen A
author_facet	Murillo,Andrea Guerrero,Carlos A Acosta,Orlando Cardozo,Carmen A
author_sort	Murillo,Andrea
title	Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
title_short	Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
title_full	Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
title_fullStr	Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
title_full_unstemmed	Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
title_sort	bone resorptive activity of osteoclast-like cells generated in vitro by peg-induced macrophage fusion
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200009
work_keys_str_mv	AT murilloandrea boneresorptiveactivityofosteoclastlikecellsgeneratedinvitrobypeginducedmacrophagefusion AT guerrerocarlosa boneresorptiveactivityofosteoclastlikecellsgeneratedinvitrobypeginducedmacrophagefusion AT acostaorlando boneresorptiveactivityofosteoclastlikecellsgeneratedinvitrobypeginducedmacrophagefusion AT cardozocarmena boneresorptiveactivityofosteoclastlikecellsgeneratedinvitrobypeginducedmacrophagefusion
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Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion

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