Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production

Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production

Objectives: The objective of this study is to determine the effects of Ethyl acetate fraction from Cudrania tricuspidata (EACT) on the interleukin-1b (IL-1b)-induced proliferation of rheumatoid synovial fbroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and p...

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Autores principales: Lee,Eun-Gyeong, Lee,Sang-ll, Chae,Han-Jung, Park,Seoung Ju, Lee,Yong Chul, Yoo,Wan-Hee
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2010
Materias:
Cudrania tricuspidata
COX
IL-1b
MMPs
PGE2
Rheumatoid Arthritis (RA)
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200010
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spelling oai:scielo:S0716-976020100002000102010-09-24Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 productionLee,Eun-GyeongLee,Sang-llChae,Han-JungPark,Seoung JuLee,Yong ChulYoo,Wan-Hee Cudrania tricuspidata COX IL-1b MMPs PGE2 Rheumatoid Arthritis (RA) Objectives: The objective of this study is to determine the effects of Ethyl acetate fraction from Cudrania tricuspidata (EACT) on the interleukin-1b (IL-1b)-induced proliferation of rheumatoid synovial fbroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and prostaglandin E2 (PGE2) by RASFs. Materials and Methods: The proliferation of RASFs was evaluated with CCK-8 reagent in the presence of IL-1b with/without EACT. The expression of MMPs, TIMP-1, COXs, PGE2 and intracellular MAPK signalings, including p-ERK, p-p38, p-JNK and NF-kB were examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA in conditions as described above. Results: EACT inhibits IL-1β-induced proliferation of RASFs and MMP-1, 3, COX-2 mRNA and protein expression, PGE2 production induced with IL-1b. EACT also inhibits the phosphorylation of ERK-1/2, p38, JNK and activation of NF-kB by IL-1b. Conclusions: These results suggest that EACT might be involved in synovial fbroblast proliferation and MMPs, COX-2, and PGE2 production, which are involved in joint destruction in rheumatoid arthritis (RA), indicating that this might be a new therapeutic modality for management of rheumatoid arthritis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.2 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200010en10.4067/S0716-97602010000200010
institution Scielo Chile
collection Scielo Chile
language English
topic Cudrania tricuspidata
COX
IL-1b
MMPs
PGE2
Rheumatoid Arthritis (RA)
spellingShingle Cudrania tricuspidata
COX
IL-1b
MMPs
PGE2
Rheumatoid Arthritis (RA)
Lee,Eun-Gyeong
Lee,Sang-ll
Chae,Han-Jung
Park,Seoung Ju
Lee,Yong Chul
Yoo,Wan-Hee
Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
description Objectives: The objective of this study is to determine the effects of Ethyl acetate fraction from Cudrania tricuspidata (EACT) on the interleukin-1b (IL-1b)-induced proliferation of rheumatoid synovial fbroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and prostaglandin E2 (PGE2) by RASFs. Materials and Methods: The proliferation of RASFs was evaluated with CCK-8 reagent in the presence of IL-1b with/without EACT. The expression of MMPs, TIMP-1, COXs, PGE2 and intracellular MAPK signalings, including p-ERK, p-p38, p-JNK and NF-kB were examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA in conditions as described above. Results: EACT inhibits IL-1β-induced proliferation of RASFs and MMP-1, 3, COX-2 mRNA and protein expression, PGE2 production induced with IL-1b. EACT also inhibits the phosphorylation of ERK-1/2, p38, JNK and activation of NF-kB by IL-1b. Conclusions: These results suggest that EACT might be involved in synovial fbroblast proliferation and MMPs, COX-2, and PGE2 production, which are involved in joint destruction in rheumatoid arthritis (RA), indicating that this might be a new therapeutic modality for management of rheumatoid arthritis.
author Lee,Eun-Gyeong
Lee,Sang-ll
Chae,Han-Jung
Park,Seoung Ju
Lee,Yong Chul
Yoo,Wan-Hee
author_facet Lee,Eun-Gyeong
Lee,Sang-ll
Chae,Han-Jung
Park,Seoung Ju
Lee,Yong Chul
Yoo,Wan-Hee
author_sort Lee,Eun-Gyeong
title Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
title_short Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
title_full Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
title_fullStr Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
title_full_unstemmed Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
title_sort ethyl acetate fraction from cudrania tricuspidata inhibts il-1β-induced rheumatoid synovial fibroblast proliferation and mmps, cox-2 and pge2 production
publisher Sociedad de Biología de Chile
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200010
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