Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens

The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a...

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Autores principales:	Graça-de Souza,Viviane K, Monteiro-Góes,Viviane, Manque,Patrício, Souza,Tatiana A.C.B, Corrêa,Paulo R.C., Buck,Gregory A, Ávila,Andréa R, Yamauchi,Lucy M, Pinge-Filho,Phileno, Goldenberg,Samuel, Krieger,Marco A, Yamada-Ogatta,Sueli F
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	Phytomonas serpens Trypanosoma cruzi antigenic cross-reactivity trypanosomatids
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011
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spelling	oai:scielo:S0716-976020100002000112010-09-24Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpensGraça-de Souza,Viviane KMonteiro-Góes,VivianeManque,PatrícioSouza,Tatiana A.C.BCorrêa,Paulo R.C.Buck,Gregory AÁvila,Andréa RYamauchi,Lucy MPinge-Filho,PhilenoGoldenberg,SamuelKrieger,Marco AYamada-Ogatta,Sueli F Phytomonas serpens Trypanosoma cruzi antigenic cross-reactivity trypanosomatids The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.2 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011en10.4067/S0716-97602010000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phytomonas serpens Trypanosoma cruzi antigenic cross-reactivity trypanosomatids
spellingShingle	Phytomonas serpens Trypanosoma cruzi antigenic cross-reactivity trypanosomatids Graça-de Souza,Viviane K Monteiro-Góes,Viviane Manque,Patrício Souza,Tatiana A.C.B Corrêa,Paulo R.C. Buck,Gregory A Ávila,Andréa R Yamauchi,Lucy M Pinge-Filho,Phileno Goldenberg,Samuel Krieger,Marco A Yamada-Ogatta,Sueli F Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
description	The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies.
author	Graça-de Souza,Viviane K Monteiro-Góes,Viviane Manque,Patrício Souza,Tatiana A.C.B Corrêa,Paulo R.C. Buck,Gregory A Ávila,Andréa R Yamauchi,Lucy M Pinge-Filho,Phileno Goldenberg,Samuel Krieger,Marco A Yamada-Ogatta,Sueli F
author_facet	Graça-de Souza,Viviane K Monteiro-Góes,Viviane Manque,Patrício Souza,Tatiana A.C.B Corrêa,Paulo R.C. Buck,Gregory A Ávila,Andréa R Yamauchi,Lucy M Pinge-Filho,Phileno Goldenberg,Samuel Krieger,Marco A Yamada-Ogatta,Sueli F
author_sort	Graça-de Souza,Viviane K
title	Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
title_short	Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
title_full	Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
title_fullStr	Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
title_full_unstemmed	Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
title_sort	sera of chagasic patients react with antigens from the tomato parasite phytomonas serpens
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011
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Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens

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