Model of chromosome associations in Mus domesticus spermatocytes

Understanding the spatial organization of the chromosomes in meiotic nuclei is crucial to our knowledge of the genome's functional regulation, stability and evolution. This study examined the nuclear architecture of Mus domesticus 2n=40 pachytene spermatocytes, analyzing the associations among...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Berríos,Soledad, Manterola,Marcia, Prieto,Zulita, López-Fenner,Julio, Page,Jesús, Fernández-Donoso,Raúl
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	bivalent associations nuclear model mouse spermatocytes heterochromatin distribution
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602010000300003
record_format	dspace
spelling	oai:scielo:S0716-976020100003000032010-11-30Model of chromosome associations in Mus domesticus spermatocytesBerríos,SoledadManterola,MarciaPrieto,ZulitaLópez-Fenner,JulioPage,JesúsFernández-Donoso,Raúl bivalent associations nuclear model mouse spermatocytes heterochromatin distribution Understanding the spatial organization of the chromosomes in meiotic nuclei is crucial to our knowledge of the genome's functional regulation, stability and evolution. This study examined the nuclear architecture of Mus domesticus 2n=40 pachytene spermatocytes, analyzing the associations among autosomal bivalents via their Centromere Telomere Complexes (CTC). The study developed a nuclear model in which each CTC was represented as a 3D computer object. The probability of a given combination of associations among CTC was estimated by simulating a random distribution of 19 indistinguishable CTC over n indistinguishable "cells" on the nuclear envelope. The estimated association frequencies resulting from this numerical approach were similar to those obtained by quantifying actual associations in pachytene spermatocyte spreads. The nuclear localization and associations of CTC through the meiotic prophase in well-preserved nuclei were also analyzed. We concluded that throughout the meiotic prophase: 1) the CTC of autosomal bivalents are not randomly distributed in the nuclear space; 2) the CTC associate amongst themselves, probably at random, over a small surface of the nuclear envelope, at the beginning of the meiotic prophase; 3) the initial aggregation of centromere regions occurring in lepto-zygotene likely resolves into several smaller aggregates according to patterns of preferential partitioning; 4) these smaller aggregates spread over the inner face of the nuclear envelope, remaining stable until advanced stages of the meiotic prophase or even until the first meiotic division.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.3 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300003en10.4067/S0716-97602010000300003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bivalent associations nuclear model mouse spermatocytes heterochromatin distribution
spellingShingle	bivalent associations nuclear model mouse spermatocytes heterochromatin distribution Berríos,Soledad Manterola,Marcia Prieto,Zulita López-Fenner,Julio Page,Jesús Fernández-Donoso,Raúl Model of chromosome associations in Mus domesticus spermatocytes
description	Understanding the spatial organization of the chromosomes in meiotic nuclei is crucial to our knowledge of the genome's functional regulation, stability and evolution. This study examined the nuclear architecture of Mus domesticus 2n=40 pachytene spermatocytes, analyzing the associations among autosomal bivalents via their Centromere Telomere Complexes (CTC). The study developed a nuclear model in which each CTC was represented as a 3D computer object. The probability of a given combination of associations among CTC was estimated by simulating a random distribution of 19 indistinguishable CTC over n indistinguishable "cells" on the nuclear envelope. The estimated association frequencies resulting from this numerical approach were similar to those obtained by quantifying actual associations in pachytene spermatocyte spreads. The nuclear localization and associations of CTC through the meiotic prophase in well-preserved nuclei were also analyzed. We concluded that throughout the meiotic prophase: 1) the CTC of autosomal bivalents are not randomly distributed in the nuclear space; 2) the CTC associate amongst themselves, probably at random, over a small surface of the nuclear envelope, at the beginning of the meiotic prophase; 3) the initial aggregation of centromere regions occurring in lepto-zygotene likely resolves into several smaller aggregates according to patterns of preferential partitioning; 4) these smaller aggregates spread over the inner face of the nuclear envelope, remaining stable until advanced stages of the meiotic prophase or even until the first meiotic division.
author	Berríos,Soledad Manterola,Marcia Prieto,Zulita López-Fenner,Julio Page,Jesús Fernández-Donoso,Raúl
author_facet	Berríos,Soledad Manterola,Marcia Prieto,Zulita López-Fenner,Julio Page,Jesús Fernández-Donoso,Raúl
author_sort	Berríos,Soledad
title	Model of chromosome associations in Mus domesticus spermatocytes
title_short	Model of chromosome associations in Mus domesticus spermatocytes
title_full	Model of chromosome associations in Mus domesticus spermatocytes
title_fullStr	Model of chromosome associations in Mus domesticus spermatocytes
title_full_unstemmed	Model of chromosome associations in Mus domesticus spermatocytes
title_sort	model of chromosome associations in mus domesticus spermatocytes
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300003
work_keys_str_mv	AT berriossoledad modelofchromosomeassociationsinmusdomesticusspermatocytes AT manterolamarcia modelofchromosomeassociationsinmusdomesticusspermatocytes AT prietozulita modelofchromosomeassociationsinmusdomesticusspermatocytes AT lopezfennerjulio modelofchromosomeassociationsinmusdomesticusspermatocytes AT pagejesus modelofchromosomeassociationsinmusdomesticusspermatocytes AT fernandezdonosoraul modelofchromosomeassociationsinmusdomesticusspermatocytes
_version_	1718441462644867072

Model of chromosome associations in Mus domesticus spermatocytes

Ejemplares similares