Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization

We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zivkovic,Irena P, Stojanovic,Marijana M, Petrusic,Vladimir Z, Inic-Kanada,Aleksandra B, Micic,Mileva V, Dimitrijevic,Ljiljana A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	Autoantibodies C57BL/6 mice hyperimmunization network
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602010000400003
record_format	dspace
spelling	oai:scielo:S0716-976020100004000032011-02-01Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunizationZivkovic,Irena PStojanovic,Marijana MPetrusic,Vladimir ZInic-Kanada,Aleksandra BMicic,Mileva VDimitrijevic,Ljiljana A Autoantibodies C57BL/6 mice hyperimmunization network We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable to anti-phospholipid antibodies; we explain that this absence results from idiotypic connectivity. Six groups of C57BL/6 mice were hyperimmunized with TTd in aluminum hydroxide or glycerol, with or without pretreatments. Pretreated mice had been injected with polyclonal or nonspecific immune stimulators, such as complete Freund's adjuvant (CFA) or glycerol. The epitope specificity of induced antibodies was tested by indirect ELISA using a tetanus toxoid immunogen and these autoantigens: phospholipids, gangliosides, laminin. Idiotypic connectivity was tested by competitive ELISA and gauged from the degree to which the interaction of idiotypic/anti-idiotypic complementary antibodies was inhibited in the presence of immunized sera antibodies. Higher idiotypic connectivity was noted amongst pretreated mice. There was a positive correlation between higher connectivity and autoantibody levels that acted to favor the participation of natural autoantibodies in the inhibitory process. We conclude that idiotypic connectivity plays a protective role in immunization-induced autoimmunity.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.4 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400003en10.4067/S0716-97602010000400003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Autoantibodies C57BL/6 mice hyperimmunization network
spellingShingle	Autoantibodies C57BL/6 mice hyperimmunization network Zivkovic,Irena P Stojanovic,Marijana M Petrusic,Vladimir Z Inic-Kanada,Aleksandra B Micic,Mileva V Dimitrijevic,Ljiljana A Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
description	We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable to anti-phospholipid antibodies; we explain that this absence results from idiotypic connectivity. Six groups of C57BL/6 mice were hyperimmunized with TTd in aluminum hydroxide or glycerol, with or without pretreatments. Pretreated mice had been injected with polyclonal or nonspecific immune stimulators, such as complete Freund's adjuvant (CFA) or glycerol. The epitope specificity of induced antibodies was tested by indirect ELISA using a tetanus toxoid immunogen and these autoantigens: phospholipids, gangliosides, laminin. Idiotypic connectivity was tested by competitive ELISA and gauged from the degree to which the interaction of idiotypic/anti-idiotypic complementary antibodies was inhibited in the presence of immunized sera antibodies. Higher idiotypic connectivity was noted amongst pretreated mice. There was a positive correlation between higher connectivity and autoantibody levels that acted to favor the participation of natural autoantibodies in the inhibitory process. We conclude that idiotypic connectivity plays a protective role in immunization-induced autoimmunity.
author	Zivkovic,Irena P Stojanovic,Marijana M Petrusic,Vladimir Z Inic-Kanada,Aleksandra B Micic,Mileva V Dimitrijevic,Ljiljana A
author_facet	Zivkovic,Irena P Stojanovic,Marijana M Petrusic,Vladimir Z Inic-Kanada,Aleksandra B Micic,Mileva V Dimitrijevic,Ljiljana A
author_sort	Zivkovic,Irena P
title	Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
title_short	Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
title_full	Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
title_fullStr	Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
title_full_unstemmed	Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
title_sort	network connectivity is shown to change in c57bl/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400003
work_keys_str_mv	AT zivkovicirenap networkconnectivityisshowntochangeinc57bl6miceduringacontinuingimmuneresponsesubsequenttotetanustoxoidhyperimmunization AT stojanovicmarijanam networkconnectivityisshowntochangeinc57bl6miceduringacontinuingimmuneresponsesubsequenttotetanustoxoidhyperimmunization AT petrusicvladimirz networkconnectivityisshowntochangeinc57bl6miceduringacontinuingimmuneresponsesubsequenttotetanustoxoidhyperimmunization AT inickanadaaleksandrab networkconnectivityisshowntochangeinc57bl6miceduringacontinuingimmuneresponsesubsequenttotetanustoxoidhyperimmunization AT micicmilevav networkconnectivityisshowntochangeinc57bl6miceduringacontinuingimmuneresponsesubsequenttotetanustoxoidhyperimmunization AT dimitrijevicljiljanaa networkconnectivityisshowntochangeinc57bl6miceduringacontinuingimmuneresponsesubsequenttotetanustoxoidhyperimmunization
_version_	1718441466437566464

Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization

Ejemplares similares