Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line

Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compoun...

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Autores principales:	Votto,Ana P. S, Domingues,Beatriz S, de Souza,Michele M, da Silva Júnior,Flavio M. R, Caldas,Sergiane S, Filgueira,Daza M. V. B, Clementin,Rosilene M, Primel,Ednei G, Vallochi,Adriana L, Furlong,Eliana B, Trindade,Gilma S
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	antioxidant apoptosis and/or necrosis DNA damage MDR phenotype onion (Allium cepa) tumoral cell
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400007
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spelling	oai:scielo:S0716-976020100004000072011-02-01Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell lineVotto,Ana P. SDomingues,Beatriz Sde Souza,Michele Mda Silva Júnior,Flavio M. RCaldas,Sergiane SFilgueira,Daza M. V. BClementin,Rosilene MPrimel,Ednei GVallochi,Adriana LFurlong,Eliana BTrindade,Gilma S antioxidant apoptosis and/or necrosis DNA damage MDR phenotype onion (Allium cepa) tumoral cell Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the Lucena cells experienced a significant increase of necrosis. An antioxidant capacity was verified for OE discarding oxidative damage. However, OE provoked similar significant DNA damage in both cell lines. Thus, the OE capacity to overcome the MDR phenotype suggests anti-MDR action of OE.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.4 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400007en10.4067/S0716-97602010000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antioxidant apoptosis and/or necrosis DNA damage MDR phenotype onion (Allium cepa) tumoral cell
spellingShingle	antioxidant apoptosis and/or necrosis DNA damage MDR phenotype onion (Allium cepa) tumoral cell Votto,Ana P. S Domingues,Beatriz S de Souza,Michele M da Silva Júnior,Flavio M. R Caldas,Sergiane S Filgueira,Daza M. V. B Clementin,Rosilene M Primel,Ednei G Vallochi,Adriana L Furlong,Eliana B Trindade,Gilma S Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
description	Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the Lucena cells experienced a significant increase of necrosis. An antioxidant capacity was verified for OE discarding oxidative damage. However, OE provoked similar significant DNA damage in both cell lines. Thus, the OE capacity to overcome the MDR phenotype suggests anti-MDR action of OE.
author	Votto,Ana P. S Domingues,Beatriz S de Souza,Michele M da Silva Júnior,Flavio M. R Caldas,Sergiane S Filgueira,Daza M. V. B Clementin,Rosilene M Primel,Ednei G Vallochi,Adriana L Furlong,Eliana B Trindade,Gilma S
author_facet	Votto,Ana P. S Domingues,Beatriz S de Souza,Michele M da Silva Júnior,Flavio M. R Caldas,Sergiane S Filgueira,Daza M. V. B Clementin,Rosilene M Primel,Ednei G Vallochi,Adriana L Furlong,Eliana B Trindade,Gilma S
author_sort	Votto,Ana P. S
title	Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
title_short	Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
title_full	Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
title_fullStr	Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
title_full_unstemmed	Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
title_sort	toxicity mechanisms of onion (allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400007
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Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line

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