Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies

7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. Fo...

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Autores principales:	Favero,Giovani M, Maranhão,Raul C, Maria,Durvanei A, Levy,Débora, Bydlowski,Sérgio P
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	emulsion LDL LDE 7-ketocholesterol oxidized LDL oxysterol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400008
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spelling	oai:scielo:S0716-976020100004000082011-02-01Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studiesFavero,Giovani MMaranhão,Raul CMaria,Durvanei ALevy,DéboraBydlowski,Sérgio P emulsion LDL LDE 7-ketocholesterol oxidized LDL oxysterol 7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cytotoxic effects of oxidized LDL have been widely attributed to bioactive compounds like oxysterols. The toxicity is in part due to 7-KC. Here we aimed to demonstrate the possibility of incorporating 7-KC into the synthetic nanoemulsion LDE, which resembles LDL in composition and behavior. This would provide a suitable artificial particle resembling LDL to study 7-KC metabolism. We were able to incorpórate 7-KC in several amounts into LDE. The incorporation was evaluated and confirmed by several methods, including gel filtration chromatography, using radiolabeled lipids. The incorporation did not change the main lipid composition characteristics of the new nanoparticle. Particle sizes were also evaluated and did not differ from LDE. In vivo studies were performed by injecting the nanoemulsion into mice. The plasma kinetics and the targeted organs were the same as described for LDE. Therefore, 7-KC-LDE maintains composition, size and some functional characteristics of LDE and could be used in experiments dealing with 7-ketocholesterol metabolism in lipoproteins.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.4 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400008en10.4067/S0716-97602010000400008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	emulsion LDL LDE 7-ketocholesterol oxidized LDL oxysterol
spellingShingle	emulsion LDL LDE 7-ketocholesterol oxidized LDL oxysterol Favero,Giovani M Maranhão,Raul C Maria,Durvanei A Levy,Débora Bydlowski,Sérgio P Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
description	7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cytotoxic effects of oxidized LDL have been widely attributed to bioactive compounds like oxysterols. The toxicity is in part due to 7-KC. Here we aimed to demonstrate the possibility of incorporating 7-KC into the synthetic nanoemulsion LDE, which resembles LDL in composition and behavior. This would provide a suitable artificial particle resembling LDL to study 7-KC metabolism. We were able to incorpórate 7-KC in several amounts into LDE. The incorporation was evaluated and confirmed by several methods, including gel filtration chromatography, using radiolabeled lipids. The incorporation did not change the main lipid composition characteristics of the new nanoparticle. Particle sizes were also evaluated and did not differ from LDE. In vivo studies were performed by injecting the nanoemulsion into mice. The plasma kinetics and the targeted organs were the same as described for LDE. Therefore, 7-KC-LDE maintains composition, size and some functional characteristics of LDE and could be used in experiments dealing with 7-ketocholesterol metabolism in lipoproteins.
author	Favero,Giovani M Maranhão,Raul C Maria,Durvanei A Levy,Débora Bydlowski,Sérgio P
author_facet	Favero,Giovani M Maranhão,Raul C Maria,Durvanei A Levy,Débora Bydlowski,Sérgio P
author_sort	Favero,Giovani M
title	Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
title_short	Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
title_full	Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
title_fullStr	Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
title_full_unstemmed	Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
title_sort	synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: in vitro and in vivo studies
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400008
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Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies

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