Divergent effects of Nitric oxide on airway epithelial cell activation

Nitric oxide (NO*) is a gaseous mediator synthesized by Nitric oxide sinthases. NO* is involved in the modulation of inflammation, but its role in airway inflammation remains controversial. We investigated the role of NO* in the synthesis of the chemok Nes Interleukin-8 and Monocyte Chemotactic Prot...

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Autores principales:	Neri,Tommaso, Conti,Ilaria, Cerri,Chiara, Tavanti,Laura, Paggiaro,Pierluigi, Celi,Alessandro
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	Adhesion molecules Airway inflammation Alveolar Epithelium Chemok Nes Nitric Oxide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400012
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spelling	oai:scielo:S0716-976020100004000122011-02-01Divergent effects of Nitric oxide on airway epithelial cell activationNeri,TommasoConti,IlariaCerri,ChiaraTavanti,LauraPaggiaro,PierluigiCeli,Alessandro Adhesion molecules Airway inflammation Alveolar Epithelium Chemok Nes Nitric Oxide Nitric oxide (NO) is a gaseous mediator synthesized by Nitric oxide sinthases. NO is involved in the modulation of inflammation, but its role in airway inflammation remains controversial. We investigated the role of NO* in the synthesis of the chemok Nes Interleukin-8 and Monocyte Chemotactic Protein-1, and of Intercellular Adhesion Molecule-1 by human airway epithelial cells. normal human bronchial epithelial cells and the bronchial epithelial cell line BEAS-2B were used. Neterleukin-8 (IL-8) and Monocyte Chemotactic Protein-1 (MCP-1) secretion and Intercellular Adhesion Molecule-1 (ICAM-1) expression were measured by ELISA. mRNA was assessed by semiquantitative RTI-PCR. Neterleukin-8 secretion was significantly reduced after 24h incubation with the NO* donor, sodium nitroprusside. The effect was dose-dependent. Similar results were obta Ned with S-Nitroso-N-D,L-penicillam Ne and S-Nitroso-L-glutathione. Inhibition of endogenous NO* with the Nitric oxide synthase inhibitor N-Nitro-L-arg N Ne-methyl-esther caused an increase in IL-8 secretion by lypopolisaccharide- and cytok Ne-stimulated BEAS-2B cells. Sodium nitroprusside also caused a reduction in Monocyte Chemotactic Protein-1 secretion by both cell types. In contrast, Intercellular Adhesion Molecule-1 expression was upregulated by sodium NItroprusside. RTI-PCR results indícate that the modulation of protein levels was paralleled by modification in mRNA levels. NO* has divergent effects on the synthesis of different inflammatory mediators in human bronchial epithelial cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.4 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400012en10.4067/S0716-97602010000400012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adhesion molecules Airway inflammation Alveolar Epithelium Chemok Nes Nitric Oxide
spellingShingle	Adhesion molecules Airway inflammation Alveolar Epithelium Chemok Nes Nitric Oxide Neri,Tommaso Conti,Ilaria Cerri,Chiara Tavanti,Laura Paggiaro,Pierluigi Celi,Alessandro Divergent effects of Nitric oxide on airway epithelial cell activation
description	Nitric oxide (NO) is a gaseous mediator synthesized by Nitric oxide sinthases. NO is involved in the modulation of inflammation, but its role in airway inflammation remains controversial. We investigated the role of NO* in the synthesis of the chemok Nes Interleukin-8 and Monocyte Chemotactic Protein-1, and of Intercellular Adhesion Molecule-1 by human airway epithelial cells. normal human bronchial epithelial cells and the bronchial epithelial cell line BEAS-2B were used. Neterleukin-8 (IL-8) and Monocyte Chemotactic Protein-1 (MCP-1) secretion and Intercellular Adhesion Molecule-1 (ICAM-1) expression were measured by ELISA. mRNA was assessed by semiquantitative RTI-PCR. Neterleukin-8 secretion was significantly reduced after 24h incubation with the NO* donor, sodium nitroprusside. The effect was dose-dependent. Similar results were obta Ned with S-Nitroso-N-D,L-penicillam Ne and S-Nitroso-L-glutathione. Inhibition of endogenous NO* with the Nitric oxide synthase inhibitor N-Nitro-L-arg N Ne-methyl-esther caused an increase in IL-8 secretion by lypopolisaccharide- and cytok Ne-stimulated BEAS-2B cells. Sodium nitroprusside also caused a reduction in Monocyte Chemotactic Protein-1 secretion by both cell types. In contrast, Intercellular Adhesion Molecule-1 expression was upregulated by sodium NItroprusside. RTI-PCR results indícate that the modulation of protein levels was paralleled by modification in mRNA levels. NO* has divergent effects on the synthesis of different inflammatory mediators in human bronchial epithelial cells.
author	Neri,Tommaso Conti,Ilaria Cerri,Chiara Tavanti,Laura Paggiaro,Pierluigi Celi,Alessandro
author_facet	Neri,Tommaso Conti,Ilaria Cerri,Chiara Tavanti,Laura Paggiaro,Pierluigi Celi,Alessandro
author_sort	Neri,Tommaso
title	Divergent effects of Nitric oxide on airway epithelial cell activation
title_short	Divergent effects of Nitric oxide on airway epithelial cell activation
title_full	Divergent effects of Nitric oxide on airway epithelial cell activation
title_fullStr	Divergent effects of Nitric oxide on airway epithelial cell activation
title_full_unstemmed	Divergent effects of Nitric oxide on airway epithelial cell activation
title_sort	divergent effects of nitric oxide on airway epithelial cell activation
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400012
work_keys_str_mv	AT neritommaso divergenteffectsofnitricoxideonairwayepithelialcellactivation AT contiilaria divergenteffectsofnitricoxideonairwayepithelialcellactivation AT cerrichiara divergenteffectsofnitricoxideonairwayepithelialcellactivation AT tavantilaura divergenteffectsofnitricoxideonairwayepithelialcellactivation AT paggiaropierluigi divergenteffectsofnitricoxideonairwayepithelialcellactivation AT celialessandro divergenteffectsofnitricoxideonairwayepithelialcellactivation
_version_	1718441468635381760

Divergent effects of Nitric oxide on airway epithelial cell activation

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