Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environme...

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Autores principales:	Hernandez,Pedro P, Undurraga,Cristian, Gallardo,Viviana E, Mackenzie,Natalia, Allende,Miguel L, Reyes,Ariel E
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	acute exposure apoptosis copper early life stage heat shock protein-70 zebrafish
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002
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spelling	oai:scielo:S0716-976020110001000022011-09-01Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvaeHernandez,Pedro PUndurraga,CristianGallardo,Viviana EMackenzie,NataliaAllende,Miguel LReyes,Ariel E acute exposure apoptosis copper early life stage heat shock protein-70 zebrafish Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration-dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002en10.4067/S0716-97602011000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	acute exposure apoptosis copper early life stage heat shock protein-70 zebrafish
spellingShingle	acute exposure apoptosis copper early life stage heat shock protein-70 zebrafish Hernandez,Pedro P Undurraga,Cristian Gallardo,Viviana E Mackenzie,Natalia Allende,Miguel L Reyes,Ariel E Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
description	Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration-dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.
author	Hernandez,Pedro P Undurraga,Cristian Gallardo,Viviana E Mackenzie,Natalia Allende,Miguel L Reyes,Ariel E
author_facet	Hernandez,Pedro P Undurraga,Cristian Gallardo,Viviana E Mackenzie,Natalia Allende,Miguel L Reyes,Ariel E
author_sort	Hernandez,Pedro P
title	Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
title_short	Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
title_full	Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
title_fullStr	Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
title_full_unstemmed	Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
title_sort	sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002
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Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

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