Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization

Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region cor...

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Autores principales:	Cohen,Débora J, Maldera,Julieta A, Weigel Muñoz,Mariana, Ernesto,Juan I, Vasen,Gustavo, Cuasnicu,Patricia S
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	sperm gamete fusion CRISP egg fertilization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004
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spelling	oai:scielo:S0716-976020110002000042011-09-20Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilizationCohen,Débora JMaldera,Julieta AWeigel Muñoz,MarianaErnesto,Juan IVasen,GustavoCuasnicu,Patricia S sperm gamete fusion CRISP egg fertilization Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2, as well as the comparison of their corresponding S2 regions, suggest that both proteins bind to common complementary sites in the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.2 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004en10.4067/S0716-97602011000200004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	sperm gamete fusion CRISP egg fertilization
spellingShingle	sperm gamete fusion CRISP egg fertilization Cohen,Débora J Maldera,Julieta A Weigel Muñoz,Mariana Ernesto,Juan I Vasen,Gustavo Cuasnicu,Patricia S Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
description	Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2, as well as the comparison of their corresponding S2 regions, suggest that both proteins bind to common complementary sites in the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.
author	Cohen,Débora J Maldera,Julieta A Weigel Muñoz,Mariana Ernesto,Juan I Vasen,Gustavo Cuasnicu,Patricia S
author_facet	Cohen,Débora J Maldera,Julieta A Weigel Muñoz,Mariana Ernesto,Juan I Vasen,Gustavo Cuasnicu,Patricia S
author_sort	Cohen,Débora J
title	Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_short	Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_full	Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_fullStr	Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_full_unstemmed	Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_sort	cysteine-rich secretory proteins (crisp) and their role in mammalian fertilization
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004
work_keys_str_mv	AT cohendeboraj cysteinerichsecretoryproteinscrispandtheirroleinmammalianfertilization AT malderajulietaa cysteinerichsecretoryproteinscrispandtheirroleinmammalianfertilization AT weigelmunozmariana cysteinerichsecretoryproteinscrispandtheirroleinmammalianfertilization AT ernestojuani cysteinerichsecretoryproteinscrispandtheirroleinmammalianfertilization AT vasengustavo cysteinerichsecretoryproteinscrispandtheirroleinmammalianfertilization AT cuasnicupatricias cysteinerichsecretoryproteinscrispandtheirroleinmammalianfertilization
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Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization

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