Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm

We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm s...

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Autores principales:	de los Reyes,Mónica, Palomino,Jaime, Martínez,Víctor, Aretio,Carolina, Gutiérrez,Michel
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	acrosin capacitation dog sperm cryopreservation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200005
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spelling	oai:scielo:S0716-976020110002000052011-09-20Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog spermde los Reyes,MónicaPalomino,JaimeMartínez,VíctorAretio,CarolinaGutiérrez,Michel acrosin capacitation dog sperm cryopreservation We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. After incubation, the samples were assessed by the indirect immunofluorescent staining technique. The probability of having unlabeled sperm (PUS), indicating acrosin loss, was modelled by a binomial distribution using logistic regression. There was a linear relationship between PUS and time at both temperatures (p<0.001); however, a major percentage of unlabeled sperm was observed in frozen/thawed samples soon after incubation, indicating that the release of acrosin was affected by capacitation time, mainly in frozen samples. Temperature influenced acrosin release only in cryopreserved sperm (p<0.05). Acrosin activity was measured by digestion halos on slides coated with gelatin-substrate film during each time period; a significant increase in the number of large halos was observed in fresh samples throughout the experiment, whereas frozen/thawed sperm showed a decreased rate of halo diameters during culture. Thus, there appears to differences between fresh and frozen dog sperm in terms of acrosin release and the level of acrosin activity in the course of in vitro capacitation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.2 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200005en10.4067/S0716-97602011000200005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	acrosin capacitation dog sperm cryopreservation
spellingShingle	acrosin capacitation dog sperm cryopreservation de los Reyes,Mónica Palomino,Jaime Martínez,Víctor Aretio,Carolina Gutiérrez,Michel Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
description	We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. After incubation, the samples were assessed by the indirect immunofluorescent staining technique. The probability of having unlabeled sperm (PUS), indicating acrosin loss, was modelled by a binomial distribution using logistic regression. There was a linear relationship between PUS and time at both temperatures (p<0.001); however, a major percentage of unlabeled sperm was observed in frozen/thawed samples soon after incubation, indicating that the release of acrosin was affected by capacitation time, mainly in frozen samples. Temperature influenced acrosin release only in cryopreserved sperm (p<0.05). Acrosin activity was measured by digestion halos on slides coated with gelatin-substrate film during each time period; a significant increase in the number of large halos was observed in fresh samples throughout the experiment, whereas frozen/thawed sperm showed a decreased rate of halo diameters during culture. Thus, there appears to differences between fresh and frozen dog sperm in terms of acrosin release and the level of acrosin activity in the course of in vitro capacitation.
author	de los Reyes,Mónica Palomino,Jaime Martínez,Víctor Aretio,Carolina Gutiérrez,Michel
author_facet	de los Reyes,Mónica Palomino,Jaime Martínez,Víctor Aretio,Carolina Gutiérrez,Michel
author_sort	de los Reyes,Mónica
title	Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
title_short	Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
title_full	Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
title_fullStr	Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
title_full_unstemmed	Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
title_sort	acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200005
work_keys_str_mv	AT delosreyesmonica acrosinreleaseandacrosinactivityduringincubationincapacitatingmediausingfreshandfrozenthaweddogsperm AT palominojaime acrosinreleaseandacrosinactivityduringincubationincapacitatingmediausingfreshandfrozenthaweddogsperm AT martinezvictor acrosinreleaseandacrosinactivityduringincubationincapacitatingmediausingfreshandfrozenthaweddogsperm AT aretiocarolina acrosinreleaseandacrosinactivityduringincubationincapacitatingmediausingfreshandfrozenthaweddogsperm AT gutierrezmichel acrosinreleaseandacrosinactivityduringincubationincapacitatingmediausingfreshandfrozenthaweddogsperm
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Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm

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