Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin

Hypobaric hypoxia is of interest due to an increase of human populations working at high altitude. Testicular damage is related to the physiological response (neoangiogenesis) to increased intrascrotal blood flow as temperature rises. Hypoxia is a stress factor with overproduction of reactive oxygen...

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Autores principales:	Vargas,Álvaro, Bustos-Obregón,Eduardo, Hartley,Ricardo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	epididymal sperm mouse ibuprofen melatonin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200008
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spelling	oai:scielo:S0716-976020110002000082011-09-20Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatoninVargas,ÁlvaroBustos-Obregón,EduardoHartley,Ricardo epididymal sperm mouse ibuprofen melatonin Hypobaric hypoxia is of interest due to an increase of human populations working at high altitude. Testicular damage is related to the physiological response (neoangiogenesis) to increased intrascrotal blood flow as temperature rises. Hypoxia is a stress factor with overproduction of reactive oxygen species (ROS). The effect of hypoxia in mice reproductive parameters is analyzed. Animals were exposed to simulated hypoxia of 4,200 meters above sea level (m.a.s.l.) in a chamber for 33.2 days, both to continuous (HH) or intermittent hypoxia (HI) with an intermittency period of 4 days hypoxia /4 days normoxia (500 m.a.s.l.). The anti-inflammatory drug Ibuprofen was administered to a group of mice to control vasodilation and increased blood flow. Melatonin was administered to another group of mice as a potent ROS scavenger. Animals in both HH and HI exposure were compared to normoxic non-treated controls. There was a hematological response in hypoxia, with an increase in hematocrit and reticulocytosis. There was also increased teratozoospermia. This damage was more pronounced in HH than HI, suggesting that alternating normoxic periods permits compensation for the effects of hypoxia. In both hypoxia systems, the level of lipoperoxidation and the instability of DNA increased. In HH, there was a reduction of teratozoospermia in melatonin-treated mice. Ibuprofen presented a protective effect on the same parameters as melatonin with both HI and HH. The quality of sperm DNA, fragmentation, unpacking and DNA stability diminished. In conclusion, reproductive damage elicited by HH or HI was partially ameliorated by simultaneous treatment with antiflogistic and/or antioxidant agents.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.2 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200008en10.4067/S0716-97602011000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	epididymal sperm mouse ibuprofen melatonin
spellingShingle	epididymal sperm mouse ibuprofen melatonin Vargas,Álvaro Bustos-Obregón,Eduardo Hartley,Ricardo Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
description	Hypobaric hypoxia is of interest due to an increase of human populations working at high altitude. Testicular damage is related to the physiological response (neoangiogenesis) to increased intrascrotal blood flow as temperature rises. Hypoxia is a stress factor with overproduction of reactive oxygen species (ROS). The effect of hypoxia in mice reproductive parameters is analyzed. Animals were exposed to simulated hypoxia of 4,200 meters above sea level (m.a.s.l.) in a chamber for 33.2 days, both to continuous (HH) or intermittent hypoxia (HI) with an intermittency period of 4 days hypoxia /4 days normoxia (500 m.a.s.l.). The anti-inflammatory drug Ibuprofen was administered to a group of mice to control vasodilation and increased blood flow. Melatonin was administered to another group of mice as a potent ROS scavenger. Animals in both HH and HI exposure were compared to normoxic non-treated controls. There was a hematological response in hypoxia, with an increase in hematocrit and reticulocytosis. There was also increased teratozoospermia. This damage was more pronounced in HH than HI, suggesting that alternating normoxic periods permits compensation for the effects of hypoxia. In both hypoxia systems, the level of lipoperoxidation and the instability of DNA increased. In HH, there was a reduction of teratozoospermia in melatonin-treated mice. Ibuprofen presented a protective effect on the same parameters as melatonin with both HI and HH. The quality of sperm DNA, fragmentation, unpacking and DNA stability diminished. In conclusion, reproductive damage elicited by HH or HI was partially ameliorated by simultaneous treatment with antiflogistic and/or antioxidant agents.
author	Vargas,Álvaro Bustos-Obregón,Eduardo Hartley,Ricardo
author_facet	Vargas,Álvaro Bustos-Obregón,Eduardo Hartley,Ricardo
author_sort	Vargas,Álvaro
title	Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
title_short	Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
title_full	Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
title_fullStr	Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
title_full_unstemmed	Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
title_sort	effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200008
work_keys_str_mv	AT vargasalvaro effectsofhypoxiaonepididymalspermparametersandprotectiveroleofibuprofenandmelatonin AT bustosobregoneduardo effectsofhypoxiaonepididymalspermparametersandprotectiveroleofibuprofenandmelatonin AT hartleyricardo effectsofhypoxiaonepididymalspermparametersandprotectiveroleofibuprofenandmelatonin
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Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin

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