Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages

The present study shows for the first time the phenolic composition and the in vitro properties (antioxidant and inhibition of nitric oxide production) of Hypericum calabricum Sprengel collected in Italy. The content of hypericins (hypericin and pseudohypericin), hyperforin, flavonoids (rutin, hyper...

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Autores principales:	Statti,Giancarlo A, Conforti,Filomena, Menichini,Federica, Marrelli,Mariangela, Gangale,Carmen, Tundis,Rosa, Loizzo,Monica R, Bonesi,Marco, Menichini,Francesco
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	antiradical activity hypericaceae Hypericum calabricum Sprengel phenolic compounds
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300001
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spelling	oai:scielo:S0716-976020110003000012011-11-07Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophagesStatti,Giancarlo AConforti,FilomenaMenichini,FedericaMarrelli,MariangelaGangale,CarmenTundis,RosaLoizzo,Monica RBonesi,MarcoMenichini,Francesco antiradical activity hypericaceae Hypericum calabricum Sprengel phenolic compounds The present study shows for the first time the phenolic composition and the in vitro properties (antioxidant and inhibition of nitric oxide production) of Hypericum calabricum Sprengel collected in Italy. The content of hypericins (hypericin and pseudohypericin), hyperforin, flavonoids (rutin, hyperoside, isoquercetrin, quercitrin, quercetin and biapigenin) and chlorogenic acid of H. calabricum, have been determined. The ethyl acetate fraction from the aerial parts of H. calabricum exhibited activity against the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 1.6 jig/ml. The test for inhibition of nitric oxide (NO) production was performed using the murine monocytic macrophage cell line RAW 264.7. The ethyl acetate fraction had significant activity with an IC50 value of 102 jig/ml and this might indicate that it would have an anti-inflammatory effect in vivo.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.3 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300001en10.4067/S0716-97602011000300001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antiradical activity hypericaceae Hypericum calabricum Sprengel phenolic compounds
spellingShingle	antiradical activity hypericaceae Hypericum calabricum Sprengel phenolic compounds Statti,Giancarlo A Conforti,Filomena Menichini,Federica Marrelli,Mariangela Gangale,Carmen Tundis,Rosa Loizzo,Monica R Bonesi,Marco Menichini,Francesco Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
description	The present study shows for the first time the phenolic composition and the in vitro properties (antioxidant and inhibition of nitric oxide production) of Hypericum calabricum Sprengel collected in Italy. The content of hypericins (hypericin and pseudohypericin), hyperforin, flavonoids (rutin, hyperoside, isoquercetrin, quercitrin, quercetin and biapigenin) and chlorogenic acid of H. calabricum, have been determined. The ethyl acetate fraction from the aerial parts of H. calabricum exhibited activity against the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 1.6 jig/ml. The test for inhibition of nitric oxide (NO) production was performed using the murine monocytic macrophage cell line RAW 264.7. The ethyl acetate fraction had significant activity with an IC50 value of 102 jig/ml and this might indicate that it would have an anti-inflammatory effect in vivo.
author	Statti,Giancarlo A Conforti,Filomena Menichini,Federica Marrelli,Mariangela Gangale,Carmen Tundis,Rosa Loizzo,Monica R Bonesi,Marco Menichini,Francesco
author_facet	Statti,Giancarlo A Conforti,Filomena Menichini,Federica Marrelli,Mariangela Gangale,Carmen Tundis,Rosa Loizzo,Monica R Bonesi,Marco Menichini,Francesco
author_sort	Statti,Giancarlo A
title	Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
title_short	Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
title_full	Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
title_fullStr	Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
title_full_unstemmed	Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
title_sort	protective effect of hypericum calabricum sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated raw 264.7 macrophages
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300001
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Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages

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