Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amaral,Marta G, Campos,Vinicius F, Seixas,Fabiana K, Cavalcanti,Paulo V, Selau,Lisiane P. R, Deschamps,Joao C, Collares,Tiago
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	TMGT DMA (N,N-dimethylacetamide) Liposome mice transgenesis histological damage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602011000300003
record_format	dspace
spelling	oai:scielo:S0716-976020110003000032011-11-07Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damageAmaral,Marta GCampos,Vinicius FSeixas,Fabiana KCavalcanti,Paulo VSelau,Lisiane P. RDeschamps,Joao CCollares,Tiago TMGT DMA (N,N-dimethylacetamide) Liposome mice transgenesis histological damage Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PC R and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological analyses demonstrated that repeated (4 times) injections of DNA complexes can affect spermatogenesis. DMSO was the most deleterious among the reagents tested. In this study, we detected the presence of transgene in the progeny, and its expression in blood cells. Consecutive injections of DNA complexes were associated with impaired spermatogenesis, suggesting requirement of optimal conditions for DNA delivery through TMGT.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.3 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003en10.4067/S0716-97602011000300003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	TMGT DMA (N,N-dimethylacetamide) Liposome mice transgenesis histological damage
spellingShingle	TMGT DMA (N,N-dimethylacetamide) Liposome mice transgenesis histological damage Amaral,Marta G Campos,Vinicius F Seixas,Fabiana K Cavalcanti,Paulo V Selau,Lisiane P. R Deschamps,Joao C Collares,Tiago Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
description	Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PC R and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological analyses demonstrated that repeated (4 times) injections of DNA complexes can affect spermatogenesis. DMSO was the most deleterious among the reagents tested. In this study, we detected the presence of transgene in the progeny, and its expression in blood cells. Consecutive injections of DNA complexes were associated with impaired spermatogenesis, suggesting requirement of optimal conditions for DNA delivery through TMGT.
author	Amaral,Marta G Campos,Vinicius F Seixas,Fabiana K Cavalcanti,Paulo V Selau,Lisiane P. R Deschamps,Joao C Collares,Tiago
author_facet	Amaral,Marta G Campos,Vinicius F Seixas,Fabiana K Cavalcanti,Paulo V Selau,Lisiane P. R Deschamps,Joao C Collares,Tiago
author_sort	Amaral,Marta G
title	Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
title_short	Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
title_full	Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
title_fullStr	Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
title_full_unstemmed	Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
title_sort	testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003
work_keys_str_mv	AT amaralmartag testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage AT camposviniciusf testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage AT seixasfabianak testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage AT cavalcantipaulov testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage AT selaulisianepr testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage AT deschampsjoaoc testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage AT collarestiago testismediatedgenetransferinmicecomparisonoftransfectionreagentsregardingtransgenetransmissionandtesticulardamage
_version_	1718441476996726784

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

Ejemplares similares