Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or...

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Autores principales: Amaral,Marta G, Campos,Vinicius F, Seixas,Fabiana K, Cavalcanti,Paulo V, Selau,Lisiane P. R, Deschamps,Joao C, Collares,Tiago
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2011
Materias:
TMGT
DMA (N,N-dimethylacetamide)
Liposome
mice
transgenesis
histological damage
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003
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