Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or...
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Auteurs principaux: | Amaral,Marta G, Campos,Vinicius F, Seixas,Fabiana K, Cavalcanti,Paulo V, Selau,Lisiane P. R, Deschamps,Joao C, Collares,Tiago |
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Langue: | English |
Publié: |
Sociedad de Biología de Chile
2011
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Sujets: | |
Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003 |
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