Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line

Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer c...

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Autores principales:	Soler,Maritza C, Molina,Jessica L, Díaz,Hugo A, Pinto,Vivian C, Barrios,Yasenka L, He,Kan, Roller,Marc, Weinstein-Oppenheimer,Caroline R
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	Cimicifuga foetida cytotoxicity Hsp-27 Immunofluorescence MCF-7 cells RT-PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005
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spelling	oai:scielo:S0716-976020110003000052011-11-07Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell lineSoler,Maritza CMolina,Jessica LDíaz,Hugo APinto,Vivian CBarrios,Yasenka LHe,KanRoller,MarcWeinstein-Oppenheimer,Caroline R Cimicifuga foetida cytotoxicity Hsp-27 Immunofluorescence MCF-7 cells RT-PCR Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine assay. In addition, the expression of Hsp-27 mRNA detected by RT-PCR and Hsp-27 protein detected by immnofluorescence was present in all conditions, except when using the highest concentration of Cimicifuga foetida extract (2,000 jig /L). We conclude that Hsp 27 expression at 2,000 jig /L Cimicifuga foetida extract is diminished. This is the first report showing the Hsp-27 expression after exposure to Cimicifuga foetida extract in MCF-7 cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.3 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005en10.4067/S0716-97602011000300005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cimicifuga foetida cytotoxicity Hsp-27 Immunofluorescence MCF-7 cells RT-PCR
spellingShingle	Cimicifuga foetida cytotoxicity Hsp-27 Immunofluorescence MCF-7 cells RT-PCR Soler,Maritza C Molina,Jessica L Díaz,Hugo A Pinto,Vivian C Barrios,Yasenka L He,Kan Roller,Marc Weinstein-Oppenheimer,Caroline R Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
description	Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine assay. In addition, the expression of Hsp-27 mRNA detected by RT-PCR and Hsp-27 protein detected by immnofluorescence was present in all conditions, except when using the highest concentration of Cimicifuga foetida extract (2,000 jig /L). We conclude that Hsp 27 expression at 2,000 jig /L Cimicifuga foetida extract is diminished. This is the first report showing the Hsp-27 expression after exposure to Cimicifuga foetida extract in MCF-7 cells.
author	Soler,Maritza C Molina,Jessica L Díaz,Hugo A Pinto,Vivian C Barrios,Yasenka L He,Kan Roller,Marc Weinstein-Oppenheimer,Caroline R
author_facet	Soler,Maritza C Molina,Jessica L Díaz,Hugo A Pinto,Vivian C Barrios,Yasenka L He,Kan Roller,Marc Weinstein-Oppenheimer,Caroline R
author_sort	Soler,Maritza C
title	Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
title_short	Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
title_full	Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
title_fullStr	Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
title_full_unstemmed	Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
title_sort	effect of the standardized cimicifuga foetida extract on hsp 27 expression in the mcf-7 cell line
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005
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Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line

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