tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori

About half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer. Chemotaxis and flagellar motility are required for colonization and persistence of H. pylori in the gastric mucus layer. It is not completely clear...

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Autores principales:	Cerda,Oscar A, Núñez-Villena,Felipe, Soto,Sarita E, Ugalde,José Manuel, López-Solís,Remigio, Toledo,Héctor
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	tlpA chemotaxis Helicobacter pylori arginine bicarbonate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300009
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spelling	oai:scielo:S0716-976020110003000092011-11-07tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pyloriCerda,Oscar ANúñez-Villena,FelipeSoto,Sarita EUgalde,José ManuelLópez-Solís,RemigioToledo,Héctor tlpA chemotaxis Helicobacter pylori arginine bicarbonate About half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer. Chemotaxis and flagellar motility are required for colonization and persistence of H. pylori in the gastric mucus layer. It is not completely clear which chemical gradients are used by H. pylori to maintain its position. TlpA, a chemotaxis receptor for arginine/ bicarbonate, has been identified. This study aimed to find out whether tlpA gene expression is required for the chemotactic response to arginine/bicarbonate. Wild-type motile H. pylori ATCC 700392 and H. pylori ATCC 43504, a strain having an interrupted tlpA gene, were used. Also, a tlpA-knockout mutant of H. pylori 700392 (H. pylori 700-tlpA::cat) was produced by homologous recombination. Expression of tlpA was assessed by a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assay. Chemotaxis was measured as a Relative Chemotaxis Response (RCR) by a modified capillary assay. H. pylori 700392 presented chemotaxis to arginine and sodium bicarbonate. H. pylori 700-tlpA::cat showed neither tlpA gene expression nor chemotaxis towards arginine and bicarbonate. Besides confirming that TlpA is a chemotactic receptor for arginine/bicarbonate in H. pylori, this study showed that tlpA gene expression is required for arginine/bicarbonate chemotaxis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.3 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300009en10.4067/S0716-97602011000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	tlpA chemotaxis Helicobacter pylori arginine bicarbonate
spellingShingle	tlpA chemotaxis Helicobacter pylori arginine bicarbonate Cerda,Oscar A Núñez-Villena,Felipe Soto,Sarita E Ugalde,José Manuel López-Solís,Remigio Toledo,Héctor tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
description	About half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer. Chemotaxis and flagellar motility are required for colonization and persistence of H. pylori in the gastric mucus layer. It is not completely clear which chemical gradients are used by H. pylori to maintain its position. TlpA, a chemotaxis receptor for arginine/ bicarbonate, has been identified. This study aimed to find out whether tlpA gene expression is required for the chemotactic response to arginine/bicarbonate. Wild-type motile H. pylori ATCC 700392 and H. pylori ATCC 43504, a strain having an interrupted tlpA gene, were used. Also, a tlpA-knockout mutant of H. pylori 700392 (H. pylori 700-tlpA::cat) was produced by homologous recombination. Expression of tlpA was assessed by a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assay. Chemotaxis was measured as a Relative Chemotaxis Response (RCR) by a modified capillary assay. H. pylori 700392 presented chemotaxis to arginine and sodium bicarbonate. H. pylori 700-tlpA::cat showed neither tlpA gene expression nor chemotaxis towards arginine and bicarbonate. Besides confirming that TlpA is a chemotactic receptor for arginine/bicarbonate in H. pylori, this study showed that tlpA gene expression is required for arginine/bicarbonate chemotaxis.
author	Cerda,Oscar A Núñez-Villena,Felipe Soto,Sarita E Ugalde,José Manuel López-Solís,Remigio Toledo,Héctor
author_facet	Cerda,Oscar A Núñez-Villena,Felipe Soto,Sarita E Ugalde,José Manuel López-Solís,Remigio Toledo,Héctor
author_sort	Cerda,Oscar A
title	tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
title_short	tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
title_full	tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
title_fullStr	tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
title_full_unstemmed	tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
title_sort	tlpa gene expression is required for arginine and bicarbonate chemotaxis in helicobacter pylori
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300009
work_keys_str_mv	AT cerdaoscara tlpageneexpressionisrequiredforarginineandbicarbonatechemotaxisinhelicobacterpylori AT nunezvillenafelipe tlpageneexpressionisrequiredforarginineandbicarbonatechemotaxisinhelicobacterpylori AT sotosaritae tlpageneexpressionisrequiredforarginineandbicarbonatechemotaxisinhelicobacterpylori AT ugaldejosemanuel tlpageneexpressionisrequiredforarginineandbicarbonatechemotaxisinhelicobacterpylori AT lopezsolisremigio tlpageneexpressionisrequiredforarginineandbicarbonatechemotaxisinhelicobacterpylori AT toledohector tlpageneexpressionisrequiredforarginineandbicarbonatechemotaxisinhelicobacterpylori
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tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori

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