Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts

The transcription factor Pax7 negatively regulates the activity of the muscle regulatory transcription factor MyoD, preventing muscle precursor cells from undergoing terminal differentiation. In this context, the ratio between Pax7 and MyoD protein levels is thought to be critical in allowing myogen...

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Autor principal:	Olguín,Hugo C
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	Pax7 caspase-3 calpains proteasome satellite cells muscle differentiation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400002
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spelling	oai:scielo:S0716-976020110004000022012-02-10Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblastsOlguín,Hugo C Pax7 caspase-3 calpains proteasome satellite cells muscle differentiation The transcription factor Pax7 negatively regulates the activity of the muscle regulatory transcription factor MyoD, preventing muscle precursor cells from undergoing terminal differentiation. In this context, the ratio between Pax7 and MyoD protein levels is thought to be critical in allowing myogenesis to proceed or to maintain the undifferentiated muscle precursor state. We have previously shown that Pax7 is subject to rapid down regulation in differentiating myoblasts, via a proteasome-dependent pathway. Here we present evidence indicating that Pax7 is also subject to caspase-3-dependent regulation. Furthermore, simultaneous inhibition of caspase-3 and proteasome activity induced further accumulation of Pax7 protein in differentiating myoblasts. These results suggest that at early stages of muscle differentiation, Pax7 levels are regulated by at least two independent mechanisms involving caspase-3 and proteasome activity.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.4 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400002en10.4067/S0716-97602011000400002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pax7 caspase-3 calpains proteasome satellite cells muscle differentiation
spellingShingle	Pax7 caspase-3 calpains proteasome satellite cells muscle differentiation Olguín,Hugo C Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
description	The transcription factor Pax7 negatively regulates the activity of the muscle regulatory transcription factor MyoD, preventing muscle precursor cells from undergoing terminal differentiation. In this context, the ratio between Pax7 and MyoD protein levels is thought to be critical in allowing myogenesis to proceed or to maintain the undifferentiated muscle precursor state. We have previously shown that Pax7 is subject to rapid down regulation in differentiating myoblasts, via a proteasome-dependent pathway. Here we present evidence indicating that Pax7 is also subject to caspase-3-dependent regulation. Furthermore, simultaneous inhibition of caspase-3 and proteasome activity induced further accumulation of Pax7 protein in differentiating myoblasts. These results suggest that at early stages of muscle differentiation, Pax7 levels are regulated by at least two independent mechanisms involving caspase-3 and proteasome activity.
author	Olguín,Hugo C
author_facet	Olguín,Hugo C
author_sort	Olguín,Hugo C
title	Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
title_short	Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
title_full	Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
title_fullStr	Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
title_full_unstemmed	Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
title_sort	regulation of pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400002
work_keys_str_mv	AT olguinhugoc regulationofpax7proteinlevelsbycaspase3andproteasomeactivityindifferentiatingmyoblasts
_version_	1718441479866679296

Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts

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