Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay

Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mu...

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Autores principales:	Ellahueñe,Manuel F, Pérez-Alzola,L Patricia, Olmedo,M Isabel
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	dominant lethal mutation ethanol genotoxicity micronucleus test
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100004
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spelling	oai:scielo:S0716-976020120001000042012-05-15Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assayEllahueñe,Manuel FPérez-Alzola,L PatriciaOlmedo,M Isabel dominant lethal mutation ethanol genotoxicity micronucleus test Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but observed MN frequencies were significantly lower than in the 38-week-old control group. This suggests a protective effect against genotoxic damage caused by aging, probably due to ethanol action as a hydroxyl radical scavenger. In the DLM assay, male mice drank ethanol at 15% or 30% (v/v) for 20 weeks. In both groups the number of dead implants was similar to the control, but there was a significant reduction in total implants, indicating a pre-implantation loss.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100004en10.4067/S0716-97602012000100004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	dominant lethal mutation ethanol genotoxicity micronucleus test
spellingShingle	dominant lethal mutation ethanol genotoxicity micronucleus test Ellahueñe,Manuel F Pérez-Alzola,L Patricia Olmedo,M Isabel Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
description	Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but observed MN frequencies were significantly lower than in the 38-week-old control group. This suggests a protective effect against genotoxic damage caused by aging, probably due to ethanol action as a hydroxyl radical scavenger. In the DLM assay, male mice drank ethanol at 15% or 30% (v/v) for 20 weeks. In both groups the number of dead implants was similar to the control, but there was a significant reduction in total implants, indicating a pre-implantation loss.
author	Ellahueñe,Manuel F Pérez-Alzola,L Patricia Olmedo,M Isabel
author_facet	Ellahueñe,Manuel F Pérez-Alzola,L Patricia Olmedo,M Isabel
author_sort	Ellahueñe,Manuel F
title	Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
title_short	Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
title_full	Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
title_fullStr	Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
title_full_unstemmed	Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
title_sort	chronic ethanol consumption in mice does not induce dna damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100004
work_keys_str_mv	AT ellahuenemanuelf chronicethanolconsumptioninmicedoesnotinducednadamageinsomaticorgermcellsevaluatedbythebonemarrowmicronucleousassayandthedominantlethalmutationassay AT perezalzolalpatricia chronicethanolconsumptioninmicedoesnotinducednadamageinsomaticorgermcellsevaluatedbythebonemarrowmicronucleousassayandthedominantlethalmutationassay AT olmedomisabel chronicethanolconsumptioninmicedoesnotinducednadamageinsomaticorgermcellsevaluatedbythebonemarrowmicronucleousassayandthedominantlethalmutationassay
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Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay

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