Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue

The mechanisms of exercise-induced fatigue have not been investigated using proteomic techniques, an approach that could improve our understanding and generate novel information regarding the effects of exercise. In this study, the proteom alterations of rat skeletal muscle were investigated during...

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Autores principales:	Zhao,Liping, Yan,Wenhui, Xiang,Heng, Wang,Xiaoyang, Qiao,Haixuan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	Proteomics exercise-induced fatigue skeletal muscle rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100010
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spelling	oai:scielo:S0716-976020120001000102012-05-15Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced FatigueZhao,LipingYan,WenhuiXiang,HengWang,XiaoyangQiao,Haixuan Proteomics exercise-induced fatigue skeletal muscle rat The mechanisms of exercise-induced fatigue have not been investigated using proteomic techniques, an approach that could improve our understanding and generate novel information regarding the effects of exercise. In this study, the proteom alterations of rat skeletal muscle were investigated during exercise-induced fatigue. The proteins were extracted from the skeletal muscle of SD rat thigh, and then analyzed by two-dimensional electrophoresis and PDQuest software. Compared to control samples, 10 significantly altered proteins were found in exercise samples, two of them were upregulated and eight of them were downregulated. These proteins were identified by MALDI TOF-MS. The two upregulated proteins were identified as MLC1 and myosin L2 (DTNB) regulatory light-chain precursors. The eight decreased proteins are Glyceraldehyde-3-phosphate Dehydrogenas (GAPDH); Beta enolase; Creatine kinase M chain (M-CK); ATP-AMP Transphosphorylase (AK1); myosin heavy chain (MHC); actin; Troponin I, fast-skeletal muscle (Troponin I fast-twitch isoform), fsTnI; Troponin T, fast-skeletal muscle isoforms (TnTF). In these proteins, four of the eight decreased proteins are related directly or indirectly to exercise induced fatigue. The other proteins represent diverse sets of proteins including enzymyes related to energy metabolism, skeletal muscle fabric protein and protein with unknown functions. They did not exhibit evident relationship with exercise-induced fatigue. Whereas the two identified increased proteins exhibit evident relationship with fatigue. These findings will help in understanding the mechanisms involved in exercise-induced fatigue.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100010en10.4067/S0716-97602012000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Proteomics exercise-induced fatigue skeletal muscle rat
spellingShingle	Proteomics exercise-induced fatigue skeletal muscle rat Zhao,Liping Yan,Wenhui Xiang,Heng Wang,Xiaoyang Qiao,Haixuan Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
description	The mechanisms of exercise-induced fatigue have not been investigated using proteomic techniques, an approach that could improve our understanding and generate novel information regarding the effects of exercise. In this study, the proteom alterations of rat skeletal muscle were investigated during exercise-induced fatigue. The proteins were extracted from the skeletal muscle of SD rat thigh, and then analyzed by two-dimensional electrophoresis and PDQuest software. Compared to control samples, 10 significantly altered proteins were found in exercise samples, two of them were upregulated and eight of them were downregulated. These proteins were identified by MALDI TOF-MS. The two upregulated proteins were identified as MLC1 and myosin L2 (DTNB) regulatory light-chain precursors. The eight decreased proteins are Glyceraldehyde-3-phosphate Dehydrogenas (GAPDH); Beta enolase; Creatine kinase M chain (M-CK); ATP-AMP Transphosphorylase (AK1); myosin heavy chain (MHC); actin; Troponin I, fast-skeletal muscle (Troponin I fast-twitch isoform), fsTnI; Troponin T, fast-skeletal muscle isoforms (TnTF). In these proteins, four of the eight decreased proteins are related directly or indirectly to exercise induced fatigue. The other proteins represent diverse sets of proteins including enzymyes related to energy metabolism, skeletal muscle fabric protein and protein with unknown functions. They did not exhibit evident relationship with exercise-induced fatigue. Whereas the two identified increased proteins exhibit evident relationship with fatigue. These findings will help in understanding the mechanisms involved in exercise-induced fatigue.
author	Zhao,Liping Yan,Wenhui Xiang,Heng Wang,Xiaoyang Qiao,Haixuan
author_facet	Zhao,Liping Yan,Wenhui Xiang,Heng Wang,Xiaoyang Qiao,Haixuan
author_sort	Zhao,Liping
title	Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
title_short	Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
title_full	Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
title_fullStr	Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
title_full_unstemmed	Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
title_sort	proteomic investigation of changes in rat skeletal muscle after exercise-induced fatigue
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100010
work_keys_str_mv	AT zhaoliping proteomicinvestigationofchangesinratskeletalmuscleafterexerciseinducedfatigue AT yanwenhui proteomicinvestigationofchangesinratskeletalmuscleafterexerciseinducedfatigue AT xiangheng proteomicinvestigationofchangesinratskeletalmuscleafterexerciseinducedfatigue AT wangxiaoyang proteomicinvestigationofchangesinratskeletalmuscleafterexerciseinducedfatigue AT qiaohaixuan proteomicinvestigationofchangesinratskeletalmuscleafterexerciseinducedfatigue
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Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue

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