In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents

Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sper...

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Autores principales:	Wu,Shao-Chin, Liu,Min
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	cigarette smoke oocyte maturation reproductive toxicity sperm capacitation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200001
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spelling	oai:scielo:S0716-976020120002000012012-08-30In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituentsWu,Shao-ChinLiu,Min cigarette smoke oocyte maturation reproductive toxicity sperm capacitation Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10-10M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at 10-10M during in vitro capacitation, murine sperm lost forward progression and were unable to show adequate hyperactivation, which is indicative of the incompletion of the capacitation process. Only sperm administrated with 3-ethylpyridine alone showed significant reduction in capacitation status, suggesting the chemical is the one responsible for disrupting sperm capacitation. Taken together, this is the first report that documents the effect of cigarette smoke components on oocyte maturation and sperm capacitation. The present findings demonstrate the adverse effects of smoke constituents of mammalian reproduction and the differences in sensitivity to smoke components between male and female gametes. Since both processes take place in the female reproductive system, our data provide new insights into deleterious consequences of maternal exposure to cigarette smoke.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200001en10.4067/S0716-97602012000200001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cigarette smoke oocyte maturation reproductive toxicity sperm capacitation
spellingShingle	cigarette smoke oocyte maturation reproductive toxicity sperm capacitation Wu,Shao-Chin Liu,Min In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
description	Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10-10M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at 10-10M during in vitro capacitation, murine sperm lost forward progression and were unable to show adequate hyperactivation, which is indicative of the incompletion of the capacitation process. Only sperm administrated with 3-ethylpyridine alone showed significant reduction in capacitation status, suggesting the chemical is the one responsible for disrupting sperm capacitation. Taken together, this is the first report that documents the effect of cigarette smoke components on oocyte maturation and sperm capacitation. The present findings demonstrate the adverse effects of smoke constituents of mammalian reproduction and the differences in sensitivity to smoke components between male and female gametes. Since both processes take place in the female reproductive system, our data provide new insights into deleterious consequences of maternal exposure to cigarette smoke.
author	Wu,Shao-Chin Liu,Min
author_facet	Wu,Shao-Chin Liu,Min
author_sort	Wu,Shao-Chin
title	In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
title_short	In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
title_full	In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
title_fullStr	In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
title_full_unstemmed	In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
title_sort	in vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200001
work_keys_str_mv	AT wushaochin invitroassessmentofreproductivetoxicityofcigarettesmokeanddeleteriousconsequencesofmaternalexposuretoitsconstituents AT liumin invitroassessmentofreproductivetoxicityofcigarettesmokeanddeleteriousconsequencesofmaternalexposuretoitsconstituents
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In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents

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