Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze

An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurin...

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Autores principales:	Mahesh,Ayyavu, Thangadurai,Devarajan, Melchias,Gabriel
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	Asteraceae Launaea sarmentosa Leaf explant Organogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200004
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spelling	oai:scielo:S0716-976020120002000042012-08-30Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex KuntzeMahesh,AyyavuThangadurai,DevarajanMelchias,Gabriel Asteraceae Launaea sarmentosa Leaf explant Organogenesis An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200004en10.4067/S0716-97602012000200004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Asteraceae Launaea sarmentosa Leaf explant Organogenesis
spellingShingle	Asteraceae Launaea sarmentosa Leaf explant Organogenesis Mahesh,Ayyavu Thangadurai,Devarajan Melchias,Gabriel Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
description	An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
author	Mahesh,Ayyavu Thangadurai,Devarajan Melchias,Gabriel
author_facet	Mahesh,Ayyavu Thangadurai,Devarajan Melchias,Gabriel
author_sort	Mahesh,Ayyavu
title	Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
title_short	Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
title_full	Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
title_fullStr	Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
title_full_unstemmed	Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
title_sort	rapid in vitro plant regeneration from leaf explants of launaea sarmentosa (willd.) sch. bip. ex kuntze
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200004
work_keys_str_mv	AT maheshayyavu rapidinvitroplantregenerationfromleafexplantsoflaunaeasarmentosawilldschbipexkuntze AT thangaduraidevarajan rapidinvitroplantregenerationfromleafexplantsoflaunaeasarmentosawilldschbipexkuntze AT melchiasgabriel rapidinvitroplantregenerationfromleafexplantsoflaunaeasarmentosawilldschbipexkuntze
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Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze

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