Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. Howe...

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Autores principales:	Xu,Huiyun, Zhang,Jian, Wu,Jiawei, Guan,Ying, Weng,Yuanyuan, Shang,Peng
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	Cytoskeleton fluid flow stress integrin-associated molecules MC3T3-E1 osteoblast-like cell line MLO-Y4 osteocyte-like cell line
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200008
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spelling	oai:scielo:S0716-976020120002000082012-08-30Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cellsXu,HuiyunZhang,JianWu,JiaweiGuan,YingWeng,YuanyuanShang,Peng Cytoskeleton fluid flow stress integrin-associated molecules MC3T3-E1 osteoblast-like cell line MLO-Y4 osteocyte-like cell line Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fluid flow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a significant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin avp3. However, there was no or limited increase observed in MC3T3-E1 osteoblast-like cells. Cell area and fiber stress formation were also markedly promoted by fluid flow only in MLO-Y4 cells. But the numbers of processes per cell remain unaffected in both cell lines.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200008en10.4067/S0716-97602012000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cytoskeleton fluid flow stress integrin-associated molecules MC3T3-E1 osteoblast-like cell line MLO-Y4 osteocyte-like cell line
spellingShingle	Cytoskeleton fluid flow stress integrin-associated molecules MC3T3-E1 osteoblast-like cell line MLO-Y4 osteocyte-like cell line Xu,Huiyun Zhang,Jian Wu,Jiawei Guan,Ying Weng,Yuanyuan Shang,Peng Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
description	Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fluid flow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a significant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin avp3. However, there was no or limited increase observed in MC3T3-E1 osteoblast-like cells. Cell area and fiber stress formation were also markedly promoted by fluid flow only in MLO-Y4 cells. But the numbers of processes per cell remain unaffected in both cell lines.
author	Xu,Huiyun Zhang,Jian Wu,Jiawei Guan,Ying Weng,Yuanyuan Shang,Peng
author_facet	Xu,Huiyun Zhang,Jian Wu,Jiawei Guan,Ying Weng,Yuanyuan Shang,Peng
author_sort	Xu,Huiyun
title	Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
title_short	Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
title_full	Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
title_fullStr	Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
title_full_unstemmed	Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
title_sort	oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in mlo-y4 cells, but not in mc3t3-e1 cells
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200008
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_version_	1718441487534915584

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

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