Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp

Here we show the cloning and characterization of a novel homolog of prepro C-RFa cDNA from Cyprinus carpió. The deduced preprohormone precursor of 115 amino acids leads to a mature bioactive peptide of 20 amino acids with identical sequence to other teleost C-RFa. Modeling of the mature C-RFa peptid...

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Autores principales:	Romero,Alex, Montefusco-Siegmund,Rodrigo, Valenzuela,Guillermo, Kausel,Gudrun, Muller,Marc, Brauchi,Sebastian, Figueroa,Jaime
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	C-RFa peptide prolactin releasing activity Cyprinus carpió
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200011
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spelling	oai:scielo:S0716-976020120002000112012-08-30Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carpRomero,AlexMontefusco-Siegmund,RodrigoValenzuela,GuillermoKausel,GudrunMuller,MarcBrauchi,SebastianFigueroa,Jaime C-RFa peptide prolactin releasing activity Cyprinus carpió Here we show the cloning and characterization of a novel homolog of prepro C-RFa cDNA from Cyprinus carpió. The deduced preprohormone precursor of 115 amino acids leads to a mature bioactive peptide of 20 amino acids with identical sequence to other teleost C-RFa. Modeling of the mature C-RFa peptide highlighted significant similarity to homologous human PrRP20, specifically the conserved amphipathic system defined by the C-terminal alpha-helix. Clearly, the synthetic C-RFa peptide stimulated prolactin release from primary cultured fish pituitary cells. For the first time, significant variation was shown in C-RFa mRNA and protein levels in the hypothalamus and pituitary between summer- and winter-acclimatized carp. Furthermore, C-RFa protein distribution in carp central nervous tissue was visualized by immunodetection in fibers and cells in hypothalamus, olfactory tract, cerebellum and pituitary stalk. In conclusion, we demonstrated the structure conservation of C-RFa in teleosts and mammals and immunopositive cells and fibers for C-RFa in brain areas. Finally, the increase of C-RFa expression suggests the participation of this hypothalamic factor in the mechanism of modulation in PRL expression in carp.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200011en10.4067/S0716-97602012000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	C-RFa peptide prolactin releasing activity Cyprinus carpió
spellingShingle	C-RFa peptide prolactin releasing activity Cyprinus carpió Romero,Alex Montefusco-Siegmund,Rodrigo Valenzuela,Guillermo Kausel,Gudrun Muller,Marc Brauchi,Sebastian Figueroa,Jaime Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
description	Here we show the cloning and characterization of a novel homolog of prepro C-RFa cDNA from Cyprinus carpió. The deduced preprohormone precursor of 115 amino acids leads to a mature bioactive peptide of 20 amino acids with identical sequence to other teleost C-RFa. Modeling of the mature C-RFa peptide highlighted significant similarity to homologous human PrRP20, specifically the conserved amphipathic system defined by the C-terminal alpha-helix. Clearly, the synthetic C-RFa peptide stimulated prolactin release from primary cultured fish pituitary cells. For the first time, significant variation was shown in C-RFa mRNA and protein levels in the hypothalamus and pituitary between summer- and winter-acclimatized carp. Furthermore, C-RFa protein distribution in carp central nervous tissue was visualized by immunodetection in fibers and cells in hypothalamus, olfactory tract, cerebellum and pituitary stalk. In conclusion, we demonstrated the structure conservation of C-RFa in teleosts and mammals and immunopositive cells and fibers for C-RFa in brain areas. Finally, the increase of C-RFa expression suggests the participation of this hypothalamic factor in the mechanism of modulation in PRL expression in carp.
author	Romero,Alex Montefusco-Siegmund,Rodrigo Valenzuela,Guillermo Kausel,Gudrun Muller,Marc Brauchi,Sebastian Figueroa,Jaime
author_facet	Romero,Alex Montefusco-Siegmund,Rodrigo Valenzuela,Guillermo Kausel,Gudrun Muller,Marc Brauchi,Sebastian Figueroa,Jaime
author_sort	Romero,Alex
title	Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
title_short	Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
title_full	Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
title_fullStr	Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
title_full_unstemmed	Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
title_sort	structurally conserved c-rfa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200011
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_version_	1718441488283598848

Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp

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