Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis

Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide. Chemotherapy response is very poor and resistance to hormone-based treatments is frequent in advances stages. Recently, tumor-initiating cells or cancer stem cells (CSCs) have been identified in several cancers, incl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Castellón,Enrique A, Valenzuela,Rodrigo, Lillo,Jorge, Castillo,Viviana, Contreras,Héctor R, Gallegos,Iván, Mercado,Alejandro, Huidobro,Christian
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	prostate cancer cancer stem cell spheres cultures
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602012000300011
record_format	dspace
spelling	oai:scielo:S0716-976020120003000112012-12-14Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasisCastellón,Enrique AValenzuela,RodrigoLillo,JorgeCastillo,VivianaContreras,Héctor RGallegos,IvánMercado,AlejandroHuidobro,Christian prostate cancer cancer stem cell spheres cultures Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide. Chemotherapy response is very poor and resistance to hormone-based treatments is frequent in advances stages. Recently, tumor-initiating cells or cancer stem cells (CSCs) have been identified in several cancers, including PCa. These cells are thought to be responsible for therapy resistance, relapse and metastasis. In the present work, enriched populations of CSCs were obtained using a mixed procedure that included differential clone-forming ability, sphere growing induction (prostatospheres) and magnetic-associated cell sorting (MACS). Also, stem marker expression was determined in PCa biopsies of different histological grades and metastasis samples. The signature for stem markers of the isolated CSCs was CD133+/CD44+/ABCG2+/ CD24-. Expression of stem markers (CD133, CD44, and ABCG2) was higher in medium Gleason biopsies than in lower and higher grades, and lymph-node and bone metastasis samples. These results suggest that the CSCs in PCa reach an important number in medium Gleason grades, when the tumor is still confined into the gland. At this stage, the surgical treatment is usually with curative intention. However, an important percentage of patients relapse after treatment. Number and signature of CSCs may be a prognosis factor for PCa recurrence.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.3 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300011en10.4067/S0716-97602012000300011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	prostate cancer cancer stem cell spheres cultures
spellingShingle	prostate cancer cancer stem cell spheres cultures Castellón,Enrique A Valenzuela,Rodrigo Lillo,Jorge Castillo,Viviana Contreras,Héctor R Gallegos,Iván Mercado,Alejandro Huidobro,Christian Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
description	Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide. Chemotherapy response is very poor and resistance to hormone-based treatments is frequent in advances stages. Recently, tumor-initiating cells or cancer stem cells (CSCs) have been identified in several cancers, including PCa. These cells are thought to be responsible for therapy resistance, relapse and metastasis. In the present work, enriched populations of CSCs were obtained using a mixed procedure that included differential clone-forming ability, sphere growing induction (prostatospheres) and magnetic-associated cell sorting (MACS). Also, stem marker expression was determined in PCa biopsies of different histological grades and metastasis samples. The signature for stem markers of the isolated CSCs was CD133+/CD44+/ABCG2+/ CD24-. Expression of stem markers (CD133, CD44, and ABCG2) was higher in medium Gleason biopsies than in lower and higher grades, and lymph-node and bone metastasis samples. These results suggest that the CSCs in PCa reach an important number in medium Gleason grades, when the tumor is still confined into the gland. At this stage, the surgical treatment is usually with curative intention. However, an important percentage of patients relapse after treatment. Number and signature of CSCs may be a prognosis factor for PCa recurrence.
author	Castellón,Enrique A Valenzuela,Rodrigo Lillo,Jorge Castillo,Viviana Contreras,Héctor R Gallegos,Iván Mercado,Alejandro Huidobro,Christian
author_facet	Castellón,Enrique A Valenzuela,Rodrigo Lillo,Jorge Castillo,Viviana Contreras,Héctor R Gallegos,Iván Mercado,Alejandro Huidobro,Christian
author_sort	Castellón,Enrique A
title	Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
title_short	Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
title_full	Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
title_fullStr	Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
title_full_unstemmed	Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
title_sort	molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different gleason grades and metastasis
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300011
work_keys_str_mv	AT castellonenriquea molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT valenzuelarodrigo molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT lillojorge molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT castilloviviana molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT contrerashectorr molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT gallegosivan molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT mercadoalejandro molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis AT huidobrochristian molecularsignatureofcancerstemcellsisolatedfromprostatecarcinomaandexpressionofstemmarkersindifferentgleasongradesandmetastasis
_version_	1718441490834784256

Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis

Ejemplares similares