Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats

Statins reduce cholesterol levels by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase and have a major place in the treatment of atherosclerotic disease. Recent studies have shown anti-inflammatory properties of statins. The purpose of this study was to evaluate the anti-inflammatory effec...

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Autores principales:	Tulek,Baykal, Kiyan,Esen, Kiyici,Aysel, Toy,Hatice, Bariskaner,Hulagu, Suerdem,Mecit
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400003
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spelling	oai:scielo:S0716-976020120004000032013-03-15Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female ratsTulek,BaykalKiyan,EsenKiyici,AyselToy,HaticeBariskaner,HulaguSuerdem,MecitStatins reduce cholesterol levels by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase and have a major place in the treatment of atherosclerotic disease. Recent studies have shown anti-inflammatory properties of statins. The purpose of this study was to evaluate the anti-inflammatory effect of simvastatin on bleomycin (BLM)-induced pulmonary fibrosis in rats. A total of 31 female Sprague-Dawley rats were divided into four groups: (1) intratracheal (IT) phosphate-buffered saline (PBS) + intraperitoneal (IP) PBS (n=7); (2) IT BLM + IP PBS (n=8); (3) IT BLM + low dose (LD) simvastatin (1 mg/kg daily, n=8); (4) IT BLM + high dose (HD) simvastatin (5 mg/kg daily, n=8). Simvastatin was administered IP for 15 days, beginning 1 day prior to IT BLM. The effect of simvastatin on pulmonary fibrosis was studied by measurements of IL-13, PDGF, IFN-γ, TGF-p1 levels in bronchoalveolar lavage (BAL) fluid and lung tissue hydroxyproline (HPL) content and by histopathological examination (Ashcroft score). BLM caused significant change in BAL fluid cytokine levels and increased both HPL content and histopathological score (p<0.001 for all). While LD simvastatin had no effect on cytokine levels, HD significantly reduced IL-13 (15.12 ±7.08 pg/ml vs. 4.43±2.34 pg/mL; p<0.05) and TGF-β1 levels (269.25 ±65.42 pg/mL vs. 131.75±32.65 pg/mL; p<0.05). Neither HD nor LD simvastatin attenuated HPL content or Ashcroft score. In conclusion, this study showed that LD simvastatin had no effect on a BLM-induced pulmonary fibrosis model, while the high dose caused partial improvement in profibrotic cytokine levels.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400003en10.4067/S0716-97602012000400003
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Statins reduce cholesterol levels by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase and have a major place in the treatment of atherosclerotic disease. Recent studies have shown anti-inflammatory properties of statins. The purpose of this study was to evaluate the anti-inflammatory effect of simvastatin on bleomycin (BLM)-induced pulmonary fibrosis in rats. A total of 31 female Sprague-Dawley rats were divided into four groups: (1) intratracheal (IT) phosphate-buffered saline (PBS) + intraperitoneal (IP) PBS (n=7); (2) IT BLM + IP PBS (n=8); (3) IT BLM + low dose (LD) simvastatin (1 mg/kg daily, n=8); (4) IT BLM + high dose (HD) simvastatin (5 mg/kg daily, n=8). Simvastatin was administered IP for 15 days, beginning 1 day prior to IT BLM. The effect of simvastatin on pulmonary fibrosis was studied by measurements of IL-13, PDGF, IFN-γ, TGF-p1 levels in bronchoalveolar lavage (BAL) fluid and lung tissue hydroxyproline (HPL) content and by histopathological examination (Ashcroft score). BLM caused significant change in BAL fluid cytokine levels and increased both HPL content and histopathological score (p<0.001 for all). While LD simvastatin had no effect on cytokine levels, HD significantly reduced IL-13 (15.12 ±7.08 pg/ml vs. 4.43±2.34 pg/mL; p<0.05) and TGF-β1 levels (269.25 ±65.42 pg/mL vs. 131.75±32.65 pg/mL; p<0.05). Neither HD nor LD simvastatin attenuated HPL content or Ashcroft score. In conclusion, this study showed that LD simvastatin had no effect on a BLM-induced pulmonary fibrosis model, while the high dose caused partial improvement in profibrotic cytokine levels.
author	Tulek,Baykal Kiyan,Esen Kiyici,Aysel Toy,Hatice Bariskaner,Hulagu Suerdem,Mecit
spellingShingle	Tulek,Baykal Kiyan,Esen Kiyici,Aysel Toy,Hatice Bariskaner,Hulagu Suerdem,Mecit Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
author_facet	Tulek,Baykal Kiyan,Esen Kiyici,Aysel Toy,Hatice Bariskaner,Hulagu Suerdem,Mecit
author_sort	Tulek,Baykal
title	Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
title_short	Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
title_full	Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
title_fullStr	Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
title_full_unstemmed	Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
title_sort	effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400003
work_keys_str_mv	AT tulekbaykal effectsofsimvastatinonbleomycininducedpulmonaryfibrosisinfemalerats AT kiyanesen effectsofsimvastatinonbleomycininducedpulmonaryfibrosisinfemalerats AT kiyiciaysel effectsofsimvastatinonbleomycininducedpulmonaryfibrosisinfemalerats AT toyhatice effectsofsimvastatinonbleomycininducedpulmonaryfibrosisinfemalerats AT bariskanerhulagu effectsofsimvastatinonbleomycininducedpulmonaryfibrosisinfemalerats AT suerdemmecit effectsofsimvastatinonbleomycininducedpulmonaryfibrosisinfemalerats
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Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats

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