Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP
Objectives: Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In...
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Sociedad de Biología de Chile
2012
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oai:scielo:S0716-976020120004000132013-03-15Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOPGeum-Hwa,LeeHyung-Ryong,KimSang-Yong,HanBidur,BhandaryDo-Sung,KimMin-Gul,KimByung-Ok,SoSun-Young,KimKyu-Sik,JoBo-Hee,LeeHee-Nam,SeoSoo-Wan,ChaeHan-Jung,Chae Gastrodia elata 4HBA GRP78 CHOP β-amyloid Objectives: Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In this study, GE and its pure components, gastrodin and 4-hydroxybenzyl alcohol (4HBA), were applied to β-amyloid-induced BV2 mouse microglial cells. Materials and Methods Cell viability was assessed by the MTT assay and Western blotting was also performed. Results: β-amyloid-induced cell death was shown to be induced time- and dose-dependently. To examine the cell death mechanism, we confirmed the involvement of ER stress signaling. C/EBP homologous protein (CHOP), a pro-apoptotic ER stress protein, was expressed at high levels but glucose-regulated protein 78 (GRP78), an anti-apoptotic ER stress protein with chaperone activity, was only slightly affected by treatment with β-amyloid. However, pretreatment with GE and its components inhibited the expression of CHOP but increased that of GRP78 in β-amyloid-treated cells. This study also showed that a single treatment with GE extracts, gastrodin, or 4HBA induced the expression of GRP78, a marker for enhanced protein folding machinery, suggesting a protective mechanism for GE against β-amyloid. Conclusions: This study reveals the protective effects of GE against β-amyloid-induced cell death, possibly through the enhancement of protein folding machinery of a representative protein, GRP78, and the regulation of CHOP in BV2 mouse microglial cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400013en10.4067/S0716-97602012000400013 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Gastrodia elata 4HBA GRP78 CHOP β-amyloid |
| spellingShingle |
Gastrodia elata 4HBA GRP78 CHOP β-amyloid Geum-Hwa,Lee Hyung-Ryong,Kim Sang-Yong,Han Bidur,Bhandary Do-Sung,Kim Min-Gul,Kim Byung-Ok,So Sun-Young,Kim Kyu-Sik,Jo Bo-Hee,Lee Hee-Nam,Seo Soo-Wan,Chae Han-Jung,Chae Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP |
| description |
Objectives: Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In this study, GE and its pure components, gastrodin and 4-hydroxybenzyl alcohol (4HBA), were applied to β-amyloid-induced BV2 mouse microglial cells. Materials and Methods Cell viability was assessed by the MTT assay and Western blotting was also performed. Results: β-amyloid-induced cell death was shown to be induced time- and dose-dependently. To examine the cell death mechanism, we confirmed the involvement of ER stress signaling. C/EBP homologous protein (CHOP), a pro-apoptotic ER stress protein, was expressed at high levels but glucose-regulated protein 78 (GRP78), an anti-apoptotic ER stress protein with chaperone activity, was only slightly affected by treatment with β-amyloid. However, pretreatment with GE and its components inhibited the expression of CHOP but increased that of GRP78 in β-amyloid-treated cells. This study also showed that a single treatment with GE extracts, gastrodin, or 4HBA induced the expression of GRP78, a marker for enhanced protein folding machinery, suggesting a protective mechanism for GE against β-amyloid. Conclusions: This study reveals the protective effects of GE against β-amyloid-induced cell death, possibly through the enhancement of protein folding machinery of a representative protein, GRP78, and the regulation of CHOP in BV2 mouse microglial cells. |
| author |
Geum-Hwa,Lee Hyung-Ryong,Kim Sang-Yong,Han Bidur,Bhandary Do-Sung,Kim Min-Gul,Kim Byung-Ok,So Sun-Young,Kim Kyu-Sik,Jo Bo-Hee,Lee Hee-Nam,Seo Soo-Wan,Chae Han-Jung,Chae |
| author_facet |
Geum-Hwa,Lee Hyung-Ryong,Kim Sang-Yong,Han Bidur,Bhandary Do-Sung,Kim Min-Gul,Kim Byung-Ok,So Sun-Young,Kim Kyu-Sik,Jo Bo-Hee,Lee Hee-Nam,Seo Soo-Wan,Chae Han-Jung,Chae |
| author_sort |
Geum-Hwa,Lee |
| title |
Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP |
| title_short |
Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP |
| title_full |
Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP |
| title_fullStr |
Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP |
| title_full_unstemmed |
Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against β-amyloid: The involvement of GRP78 and CHOP |
| title_sort |
gastrodia elata blume and its pure compounds protect bv-2 microglial-derived cell lines against β-amyloid: the involvement of grp78 and chop |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2012 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400013 |
| work_keys_str_mv |
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| _version_ |
1718441495074177024 |