Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro

Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 µM) or DDVP + vitamin C (VC; 10 µM) or...

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Autores principales:	Eroglu,Sema, Pandir,Dilek, Uzun,Fatma G, Bas,Hatice
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	antioxidant enzyme dichlorvos oxidative stress vitamin malondialdehyde
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100005
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spelling	oai:scielo:S0716-976020130001000052014-09-08Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitroEroglu,SemaPandir,DilekUzun,Fatma GBas,Hatice antioxidant enzyme dichlorvos oxidative stress vitamin malondialdehyde Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 µM) or DDVP + vitamin C (VC; 10 µM) or vitamin E (VE; 30 µM), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical differences between all groups for 1 µM concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P < 0.05). Groups treated with vitamins and DDVP showed protective effects of vitamins against DDVP-induced changes in antioxidant enzyme activity and lipid peroxidation (LPO) (10 µM). At 100 µM concentration of DDVP vitamins had no effect on DDVP-induced toxicity. The results show that administration of DDVP resulted in the induction of erythrocyte LPO and alterations in antioxidant enzyme activities, suggesting that reactive oxygen species (ROS) may be involved in the toxic effects of DDVP. Also the data show that the plasma level of VC and VE may ameliorate OP-induced oxidative stress by decreasing LPO in erythrocytes at certain doses of OP pesicides.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.1 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100005en10.4067/S0716-97602013000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antioxidant enzyme dichlorvos oxidative stress vitamin malondialdehyde
spellingShingle	antioxidant enzyme dichlorvos oxidative stress vitamin malondialdehyde Eroglu,Sema Pandir,Dilek Uzun,Fatma G Bas,Hatice Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
description	Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 µM) or DDVP + vitamin C (VC; 10 µM) or vitamin E (VE; 30 µM), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical differences between all groups for 1 µM concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P < 0.05). Groups treated with vitamins and DDVP showed protective effects of vitamins against DDVP-induced changes in antioxidant enzyme activity and lipid peroxidation (LPO) (10 µM). At 100 µM concentration of DDVP vitamins had no effect on DDVP-induced toxicity. The results show that administration of DDVP resulted in the induction of erythrocyte LPO and alterations in antioxidant enzyme activities, suggesting that reactive oxygen species (ROS) may be involved in the toxic effects of DDVP. Also the data show that the plasma level of VC and VE may ameliorate OP-induced oxidative stress by decreasing LPO in erythrocytes at certain doses of OP pesicides.
author	Eroglu,Sema Pandir,Dilek Uzun,Fatma G Bas,Hatice
author_facet	Eroglu,Sema Pandir,Dilek Uzun,Fatma G Bas,Hatice
author_sort	Eroglu,Sema
title	Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
title_short	Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
title_full	Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
title_fullStr	Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
title_full_unstemmed	Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
title_sort	protective role of vitamins c and e in diclorvos-induced oxidative stress in human erythrocytes in vitro
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100005
work_keys_str_mv	AT eroglusema protectiveroleofvitaminscandeindiclorvosinducedoxidativestressinhumanerythrocytesinvitro AT pandirdilek protectiveroleofvitaminscandeindiclorvosinducedoxidativestressinhumanerythrocytesinvitro AT uzunfatmag protectiveroleofvitaminscandeindiclorvosinducedoxidativestressinhumanerythrocytesinvitro AT bashatice protectiveroleofvitaminscandeindiclorvosinducedoxidativestressinhumanerythrocytesinvitro
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Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro

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