A simple and effective pressure culture system modified from a transwell cell culture system
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system base...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad de Biología de Chile
2013
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0716-97602013000100007 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0716-976020130001000072014-09-08A simple and effective pressure culture system modified from a transwell cell culture systemSun,XuerongLiu,XinguangZhang,YuehongKuang,XielanLv,BoGe,Jian Mechanical pressure Cell culture Transwell culture system cytoskeleton Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.1 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007en10.4067/S0716-97602013000100007 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Mechanical pressure Cell culture Transwell culture system cytoskeleton |
| spellingShingle |
Mechanical pressure Cell culture Transwell culture system cytoskeleton Sun,Xuerong Liu,Xinguang Zhang,Yuehong Kuang,Xielan Lv,Bo Ge,Jian A simple and effective pressure culture system modified from a transwell cell culture system |
| description |
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment. |
| author |
Sun,Xuerong Liu,Xinguang Zhang,Yuehong Kuang,Xielan Lv,Bo Ge,Jian |
| author_facet |
Sun,Xuerong Liu,Xinguang Zhang,Yuehong Kuang,Xielan Lv,Bo Ge,Jian |
| author_sort |
Sun,Xuerong |
| title |
A simple and effective pressure culture system modified from a transwell cell culture system |
| title_short |
A simple and effective pressure culture system modified from a transwell cell culture system |
| title_full |
A simple and effective pressure culture system modified from a transwell cell culture system |
| title_fullStr |
A simple and effective pressure culture system modified from a transwell cell culture system |
| title_full_unstemmed |
A simple and effective pressure culture system modified from a transwell cell culture system |
| title_sort |
simple and effective pressure culture system modified from a transwell cell culture system |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007 |
| work_keys_str_mv |
AT sunxuerong asimpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT liuxinguang asimpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT zhangyuehong asimpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT kuangxielan asimpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT lvbo asimpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT gejian asimpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT sunxuerong simpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT liuxinguang simpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT zhangyuehong simpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT kuangxielan simpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT lvbo simpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem AT gejian simpleandeffectivepressureculturesystemmodifiedfromatranswellcellculturesystem |
| _version_ |
1718441496726732800 |