Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)

We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spa...

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Autores principales:	Leonova,Olga G, Karajan,Bella P, Ivlev,Yuri F, Ivanova,Julia L, Skarlato,Sergei O, Popenko,Vladimir I
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	3D reconstruction ciliates electron microscopy macronucleus nucleoli
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010
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spelling	oai:scielo:S0716-976020130001000102014-09-08Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)Leonova,Olga GKarajan,Bella PIvlev,Yuri FIvanova,Julia LSkarlato,Sergei OPopenko,Vladimir I 3D reconstruction ciliates electron microscopy macronucleus nucleoli We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spatial distribution of nucleolar chromatin structures in these convoluted nucleoli. There are no "classical" fibrillar centers in D.nasutum nucleoli. The spatial distribution of nucleolar chromatin bodies, which play the role of nucleolar organizers in the macronucleus of D.nasutum, was studied using 3D reconstructions based on serial ultrathin sections. The relative number of nucleolar chromatin bodies was determined in macronuclei of recently fed, starved D.nasutum cells and in resting cysts. This parameter is shown to correlate with the activity of the nucleolus. However, the relative number of nucleolar chromatin bodies in different regions of the same convoluted nucleolus is approximately the same. This finding suggests equal activity in different parts of the nucleolar domain and indicates the existence of some molecular mechanism enabling it to synchronize this activity in D. nasutum nucleoli. Our data show that D. nasutum nucleoli display bipartite structure. All nucleolar chromatin bodies are shown to be located outside of nucleoli, at the periphery of the fibrillar component.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.1 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010en10.4067/S0716-97602013000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	3D reconstruction ciliates electron microscopy macronucleus nucleoli
spellingShingle	3D reconstruction ciliates electron microscopy macronucleus nucleoli Leonova,Olga G Karajan,Bella P Ivlev,Yuri F Ivanova,Julia L Skarlato,Sergei O Popenko,Vladimir I Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
description	We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spatial distribution of nucleolar chromatin structures in these convoluted nucleoli. There are no "classical" fibrillar centers in D.nasutum nucleoli. The spatial distribution of nucleolar chromatin bodies, which play the role of nucleolar organizers in the macronucleus of D.nasutum, was studied using 3D reconstructions based on serial ultrathin sections. The relative number of nucleolar chromatin bodies was determined in macronuclei of recently fed, starved D.nasutum cells and in resting cysts. This parameter is shown to correlate with the activity of the nucleolus. However, the relative number of nucleolar chromatin bodies in different regions of the same convoluted nucleolus is approximately the same. This finding suggests equal activity in different parts of the nucleolar domain and indicates the existence of some molecular mechanism enabling it to synchronize this activity in D. nasutum nucleoli. Our data show that D. nasutum nucleoli display bipartite structure. All nucleolar chromatin bodies are shown to be located outside of nucleoli, at the periphery of the fibrillar component.
author	Leonova,Olga G Karajan,Bella P Ivlev,Yuri F Ivanova,Julia L Skarlato,Sergei O Popenko,Vladimir I
author_facet	Leonova,Olga G Karajan,Bella P Ivlev,Yuri F Ivanova,Julia L Skarlato,Sergei O Popenko,Vladimir I
author_sort	Leonova,Olga G
title	Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
title_short	Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
title_full	Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
title_fullStr	Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
title_full_unstemmed	Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
title_sort	quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of didinium nasutum (ciliophora)
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010
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Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)

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