Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México

We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphyll...

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Autores principales:	Villa-Ruano,Nemesio, Zurita-Vásquez,Guilibaldo G, Pacheco-Hernández,Yesenia, Betancourt-Jiménez,Martha G, Cruz-Durán,Ramiro, Duque-Bautista,Horacio
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	Phytotherapy anti-obesity antioxidant Oaxacan ethnobotany
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200006
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spelling	oai:scielo:S0716-976020130002000062013-08-19Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, MéxicoVilla-Ruano,NemesioZurita-Vásquez,Guilibaldo GPacheco-Hernández,YeseniaBetancourt-Jiménez,Martha GCruz-Durán,RamiroDuque-Bautista,Horacio Phytotherapy anti-obesity antioxidant Oaxacan ethnobotany We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphylla, Brungmansia suaveolens and Cuphea aequipetala showed up to 60% PPL inhibition. The effect of these extracts on the kinetic parameters of PPL (Km= 0.36 mM, and Vmax=0.085 mM min -1) revealed that the alcoholic preparations of S. erianthum and C. aequipetala engendered a non-competitive inhibition (Vmax=0.055 mM min -1; Vmax= 0.053 mM min -1), whereas those of S. microphylla and B. suaveolens produced a mixed inhibition (Km= 0.567 mM, Vmax=0.051 mM min _1; Km=0.643 mM, Vmax= 0.042 mM min ¹). In addition to these findings, seven extracts from different plants were able to inhibit PPL in the range of 30-50%. Antioxidant tests against 2,2-Diphenyl-1-picryl hydrazyl (DPPH) confirmed that Arctostaphylos pungens, Gnaphalium roseum, Crotalaria pumila, Cuphea aequipetala, Rhus chondroloma, and Satureja laevigata possess relevant antioxidant activity (IC(5)0=50-80 μg mL¹). The general composition of the most effective ethanolic extracts was obtained in order to confirm their known chemistry reported by previous works. Comprehensive chemical analysis of the ethanolic extracts and their poisoning effects suggests that S. microphylla, C. aequipetala and A. pungens could be considered as the best sources with both desired properties.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.2 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200006en10.4067/S0716-97602013000200006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phytotherapy anti-obesity antioxidant Oaxacan ethnobotany
spellingShingle	Phytotherapy anti-obesity antioxidant Oaxacan ethnobotany Villa-Ruano,Nemesio Zurita-Vásquez,Guilibaldo G Pacheco-Hernández,Yesenia Betancourt-Jiménez,Martha G Cruz-Durán,Ramiro Duque-Bautista,Horacio Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
description	We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphylla, Brungmansia suaveolens and Cuphea aequipetala showed up to 60% PPL inhibition. The effect of these extracts on the kinetic parameters of PPL (Km= 0.36 mM, and Vmax=0.085 mM min -1) revealed that the alcoholic preparations of S. erianthum and C. aequipetala engendered a non-competitive inhibition (Vmax=0.055 mM min -1; Vmax= 0.053 mM min -1), whereas those of S. microphylla and B. suaveolens produced a mixed inhibition (Km= 0.567 mM, Vmax=0.051 mM min _1; Km=0.643 mM, Vmax= 0.042 mM min ¹). In addition to these findings, seven extracts from different plants were able to inhibit PPL in the range of 30-50%. Antioxidant tests against 2,2-Diphenyl-1-picryl hydrazyl (DPPH) confirmed that Arctostaphylos pungens, Gnaphalium roseum, Crotalaria pumila, Cuphea aequipetala, Rhus chondroloma, and Satureja laevigata possess relevant antioxidant activity (IC(5)0=50-80 μg mL¹). The general composition of the most effective ethanolic extracts was obtained in order to confirm their known chemistry reported by previous works. Comprehensive chemical analysis of the ethanolic extracts and their poisoning effects suggests that S. microphylla, C. aequipetala and A. pungens could be considered as the best sources with both desired properties.
author	Villa-Ruano,Nemesio Zurita-Vásquez,Guilibaldo G Pacheco-Hernández,Yesenia Betancourt-Jiménez,Martha G Cruz-Durán,Ramiro Duque-Bautista,Horacio
author_facet	Villa-Ruano,Nemesio Zurita-Vásquez,Guilibaldo G Pacheco-Hernández,Yesenia Betancourt-Jiménez,Martha G Cruz-Durán,Ramiro Duque-Bautista,Horacio
author_sort	Villa-Ruano,Nemesio
title	Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
title_short	Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
title_full	Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
title_fullStr	Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
title_full_unstemmed	Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
title_sort	anti-iipase and antioxidant properties of 30 medicinal plants used in oaxaca, méxico
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200006
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Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México

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