mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia

This study analyzed the time dependence decay of the mRNA of selected genes important for the hypoxia response. The genes chosen were the two isoforms of hypoxia-inducible factors, the three isoforms of the prolyl hydroxylase domain protein, the vascular endothelial growth factor and endothelial nit...

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Autores principales: Panariti,Alice, Miserocchi,Giuseppe, Rivolta,Ilaria
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2013
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200008
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spelling oai:scielo:S0716-976020130002000082013-08-19mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxiaPanariti,AliceMiserocchi,GiuseppeRivolta,Ilaria chronic hypoxia Hypoxia-inducible Factor Prolyl Hydroxylase Domain proteins lung RT-PCR This study analyzed the time dependence decay of the mRNA of selected genes important for the hypoxia response. The genes chosen were the two isoforms of hypoxia-inducible factors, the three isoforms of the prolyl hydroxylase domain protein, the vascular endothelial growth factor and endothelial nitric oxide synthase. mRNA and proteins were extracted from lungs obtained from control, hypoxic and 15 minutes normoxic recovered rats and analyzed by Real-time RT-PCR or by the Western Blot technique. Results indicated that in normoxia isoform 2á was the more represented hypoxia-inducible factor mRNA, and among the prolyl hydroxylase domain transcripts, isoform 3 was the least abundant. Moreover, in chronic hypoxia only hypoxia-inducible factor 1α and prolyl hydroxylase domain protein 3 increased significantly, while after 15 minutes of recovery all the mRNAs tested were decreased except endothelial nitric oxide synthase mRNA. In terms of proteins, hypoxia-inducible 1α was the isoform more significant in the nucleus, while 2á predominated in the cytosol. While the former was steady even after a brief recovery from hypoxia, the latter underwent a strong degradation. In conclusion we showed the relevance of the decay in the mRNA and protein levels upon re-oxygenation in normoxia. We believe that this has to be considered in research studies dealing with recovery from hypoxia.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.2 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200008en10.4067/S0716-97602013000200008
institution Scielo Chile
collection Scielo Chile
language English
topic chronic hypoxia
Hypoxia-inducible Factor
Prolyl Hydroxylase Domain proteins
lung
RT-PCR
spellingShingle chronic hypoxia
Hypoxia-inducible Factor
Prolyl Hydroxylase Domain proteins
lung
RT-PCR
Panariti,Alice
Miserocchi,Giuseppe
Rivolta,Ilaria
mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
description This study analyzed the time dependence decay of the mRNA of selected genes important for the hypoxia response. The genes chosen were the two isoforms of hypoxia-inducible factors, the three isoforms of the prolyl hydroxylase domain protein, the vascular endothelial growth factor and endothelial nitric oxide synthase. mRNA and proteins were extracted from lungs obtained from control, hypoxic and 15 minutes normoxic recovered rats and analyzed by Real-time RT-PCR or by the Western Blot technique. Results indicated that in normoxia isoform 2á was the more represented hypoxia-inducible factor mRNA, and among the prolyl hydroxylase domain transcripts, isoform 3 was the least abundant. Moreover, in chronic hypoxia only hypoxia-inducible factor 1α and prolyl hydroxylase domain protein 3 increased significantly, while after 15 minutes of recovery all the mRNAs tested were decreased except endothelial nitric oxide synthase mRNA. In terms of proteins, hypoxia-inducible 1α was the isoform more significant in the nucleus, while 2á predominated in the cytosol. While the former was steady even after a brief recovery from hypoxia, the latter underwent a strong degradation. In conclusion we showed the relevance of the decay in the mRNA and protein levels upon re-oxygenation in normoxia. We believe that this has to be considered in research studies dealing with recovery from hypoxia.
author Panariti,Alice
Miserocchi,Giuseppe
Rivolta,Ilaria
author_facet Panariti,Alice
Miserocchi,Giuseppe
Rivolta,Ilaria
author_sort Panariti,Alice
title mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
title_short mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
title_full mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
title_fullStr mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
title_full_unstemmed mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
title_sort mrna expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
publisher Sociedad de Biología de Chile
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200008
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AT miserocchigiuseppe mrnaexpressionprofileofselectedoxygensensinggenesinthelungduringrecoveryfromchronichypoxia
AT rivoltailaria mrnaexpressionprofileofselectedoxygensensinggenesinthelungduringrecoveryfromchronichypoxia
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