Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)

The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demo...

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Autores principales:	Gao,Zhengquan, Meng,Chunxiao, Gao,Hongzheng, Zhang,Xiaowen, Xu,Dong, Su,Yuanfeng, Wang,Yuanyuan, Zhao,Yuren, Ye,Naihao
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	Haematococcus pluvialis astaxanthin 2, 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR)
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012
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spelling	oai:scielo:S0716-976020130002000122013-08-19Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)Gao,ZhengquanMeng,ChunxiaoGao,HongzhengZhang,XiaowenXu,DongSu,YuanfengWang,YuanyuanZhao,YurenYe,Naihao Haematococcus pluvialis astaxanthin 2, 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR) The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.2 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012en10.4067/S0716-97602013000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Haematococcus pluvialis astaxanthin 2, 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR)
spellingShingle	Haematococcus pluvialis astaxanthin 2, 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR) Gao,Zhengquan Meng,Chunxiao Gao,Hongzheng Zhang,Xiaowen Xu,Dong Su,Yuanfeng Wang,Yuanyuan Zhao,Yuren Ye,Naihao Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
description	The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.
author	Gao,Zhengquan Meng,Chunxiao Gao,Hongzheng Zhang,Xiaowen Xu,Dong Su,Yuanfeng Wang,Yuanyuan Zhao,Yuren Ye,Naihao
author_facet	Gao,Zhengquan Meng,Chunxiao Gao,Hongzheng Zhang,Xiaowen Xu,Dong Su,Yuanfeng Wang,Yuanyuan Zhao,Yuren Ye,Naihao
author_sort	Gao,Zhengquan
title	Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
title_short	Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
title_full	Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
title_fullStr	Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
title_full_unstemmed	Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
title_sort	analysis of mrna expression profiles of carotenogenesis and astaxanthin production of haematococcus pluvialis under exogenous 2, 4-epibrassinolide (ebr)
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012
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Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)

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