Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients
Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on different cell surface components and modulate a variety of cellular processes. A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as...
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Sociedad de Biología de Chile
2013
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oai:scielo:S0716-976020130003000082014-01-20Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patientsVicuña,LucasPardo,EvelynCurkovic,CristóbalDoger,RemziyeOyanadel,ClaudiaMetz,ClaudiaMassardo,LoretoGonzález,AlfonsoSoza,Andrea Galectin-8 LFA-1 ICAM-1 SLE autoantibodies Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on different cell surface components and modulate a variety of cellular processes. A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as well as the presence of anti-Gal-8 autoantibodies in the prototypic autoimmune disease systemic lupus erythematosus (SLE). We have previously described that Gal-8 induces apoptosis in activated T cells interacting with certain β1 integrins and this effect is counteracted by the anti-Gal-8 autoantibodies. Given that Gal-8 can potentially interact with several glycoproteins, here we analyzed the β2 integrin Lymphocyte Function-Associated Antigen-1 (LFA-1), which is involved in leukocyte cell adhesion and immunological synapses. We show by GST-pull down assays that Gal-8 interacts with LFA-1 and this interaction is inhibited by anti-Gal-8 autoantibodies isolated from SLE patients. In cell adhesion assays, Gal-8 precluded the interaction of LFA-1 with its ligand Intracellular Adhesion Molecule-1 (ICAM-1). These results suggest that Gal-8 can exert immunosuppressive action not only by inducing apoptosis in activated T cells but also by negatively modulating the crucial function of LFA-1 in the immune system, while function-blocking autoantibodies counteract these effects.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.3 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300008en10.4067/S0716-97602013000300008 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Galectin-8 LFA-1 ICAM-1 SLE autoantibodies |
| spellingShingle |
Galectin-8 LFA-1 ICAM-1 SLE autoantibodies Vicuña,Lucas Pardo,Evelyn Curkovic,Cristóbal Doger,Remziye Oyanadel,Claudia Metz,Claudia Massardo,Loreto González,Alfonso Soza,Andrea Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients |
| description |
Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on different cell surface components and modulate a variety of cellular processes. A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as well as the presence of anti-Gal-8 autoantibodies in the prototypic autoimmune disease systemic lupus erythematosus (SLE). We have previously described that Gal-8 induces apoptosis in activated T cells interacting with certain β1 integrins and this effect is counteracted by the anti-Gal-8 autoantibodies. Given that Gal-8 can potentially interact with several glycoproteins, here we analyzed the β2 integrin Lymphocyte Function-Associated Antigen-1 (LFA-1), which is involved in leukocyte cell adhesion and immunological synapses. We show by GST-pull down assays that Gal-8 interacts with LFA-1 and this interaction is inhibited by anti-Gal-8 autoantibodies isolated from SLE patients. In cell adhesion assays, Gal-8 precluded the interaction of LFA-1 with its ligand Intracellular Adhesion Molecule-1 (ICAM-1). These results suggest that Gal-8 can exert immunosuppressive action not only by inducing apoptosis in activated T cells but also by negatively modulating the crucial function of LFA-1 in the immune system, while function-blocking autoantibodies counteract these effects. |
| author |
Vicuña,Lucas Pardo,Evelyn Curkovic,Cristóbal Doger,Remziye Oyanadel,Claudia Metz,Claudia Massardo,Loreto González,Alfonso Soza,Andrea |
| author_facet |
Vicuña,Lucas Pardo,Evelyn Curkovic,Cristóbal Doger,Remziye Oyanadel,Claudia Metz,Claudia Massardo,Loreto González,Alfonso Soza,Andrea |
| author_sort |
Vicuña,Lucas |
| title |
Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients |
| title_short |
Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients |
| title_full |
Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients |
| title_fullStr |
Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients |
| title_full_unstemmed |
Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients |
| title_sort |
galectin-8 binds to lfa-1, blocks its interaction with icam-1 and is counteracted by anti-gal-8 autoantibodies isolated from lupus patients |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300008 |
| work_keys_str_mv |
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