Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats
Objective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth....
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Sociedad de Biología de Chile
2013
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oai:scielo:S0716-976020130003000092014-01-20Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ ratsVargas Guerrero,BelindaGarcía López,Pedro MGonzález Santiago,Ana EDomínguez Rosales,José AGurrola Díaz,Carmen M chronic hyperglycemia in vivo diabetes models lns-1 gene type 2 diabetes Objective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth. In this study, we assessed lns-1 gene expression and tissue insulin levels as well as serum concentration of glucose and insulin, insulin resistance, and histological changes of the islets of Langerhans in n5-STZ rats after 20-weeks post-induction. Methods: Wistar rat pups were randomly distributed into a control group and a streptozotocin-induced group. Experimental induction involved a single intraperitoneal injection of streptozotocin (150 mg/kg) into neonates at five days after birth. Results: At 20 weeks post-induction, streptozotocin-induced rats exhibited increased serum glucose levels, reduced serum insulin levels, impaired glucose metabolism and insulin resistance compared to control rats. Histologically, streptozotocin-induced rats exhibited atrophic islets, vacuolization, and significantly fewer insulin-positive cells. lns-1 gene expression was significantly decreased in n5-STZ rats in comparison to the control group. Conclusion: Our findings support that the n5-STZ model 20 weeks post-induction represents an appropriate experimental tool to study T2D and to evaluate novel therapeutic agents and targets that involve insulin gene expression and secretion, as well as complications caused by chronic diabetes.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.3 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300009en10.4067/S0716-97602013000300009 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
chronic hyperglycemia in vivo diabetes models lns-1 gene type 2 diabetes |
| spellingShingle |
chronic hyperglycemia in vivo diabetes models lns-1 gene type 2 diabetes Vargas Guerrero,Belinda García López,Pedro M González Santiago,Ana E Domínguez Rosales,José A Gurrola Díaz,Carmen M Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| description |
Objective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth. In this study, we assessed lns-1 gene expression and tissue insulin levels as well as serum concentration of glucose and insulin, insulin resistance, and histological changes of the islets of Langerhans in n5-STZ rats after 20-weeks post-induction. Methods: Wistar rat pups were randomly distributed into a control group and a streptozotocin-induced group. Experimental induction involved a single intraperitoneal injection of streptozotocin (150 mg/kg) into neonates at five days after birth. Results: At 20 weeks post-induction, streptozotocin-induced rats exhibited increased serum glucose levels, reduced serum insulin levels, impaired glucose metabolism and insulin resistance compared to control rats. Histologically, streptozotocin-induced rats exhibited atrophic islets, vacuolization, and significantly fewer insulin-positive cells. lns-1 gene expression was significantly decreased in n5-STZ rats in comparison to the control group. Conclusion: Our findings support that the n5-STZ model 20 weeks post-induction represents an appropriate experimental tool to study T2D and to evaluate novel therapeutic agents and targets that involve insulin gene expression and secretion, as well as complications caused by chronic diabetes. |
| author |
Vargas Guerrero,Belinda García López,Pedro M González Santiago,Ana E Domínguez Rosales,José A Gurrola Díaz,Carmen M |
| author_facet |
Vargas Guerrero,Belinda García López,Pedro M González Santiago,Ana E Domínguez Rosales,José A Gurrola Díaz,Carmen M |
| author_sort |
Vargas Guerrero,Belinda |
| title |
Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_short |
Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_full |
Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_fullStr |
Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_full_unstemmed |
Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_sort |
reduction of lns-1 gene expression and tissue insulin levels in n5-stz rats |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300009 |
| work_keys_str_mv |
AT vargasguerrerobelinda reductionoflns1geneexpressionandtissueinsulinlevelsinn5stzrats AT garcialopezpedrom reductionoflns1geneexpressionandtissueinsulinlevelsinn5stzrats AT gonzalezsantiagoanae reductionoflns1geneexpressionandtissueinsulinlevelsinn5stzrats AT dominguezrosalesjosea reductionoflns1geneexpressionandtissueinsulinlevelsinn5stzrats AT gurroladiazcarmenm reductionoflns1geneexpressionandtissueinsulinlevelsinn5stzrats |
| _version_ |
1718441503533039616 |