Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli

Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousan...

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Autores principales:	Muñoz,Yorka, Fuenzalida,Karen, Bronfman,Miguel, Gatica,Arnaldo, Sepúlveda,Marcelo, Bacigalupo,Juan, Roth,Alejandro D, Delgado,Ricardo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	Rhabdomere Drosophila microvilli phototransduction TRP channel fatty acid analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010
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spelling	oai:scielo:S0716-976020130003000102014-01-20Fatty acid composition of Drosophila photoreceptor light-sensitive microvilliMuñoz,YorkaFuenzalida,KarenBronfman,MiguelGatica,ArnaldoSepúlveda,MarceloBacigalupo,JuanRoth,Alejandro DDelgado,Ricardo Rhabdomere Drosophila microvilli phototransduction TRP channel fatty acid analysis Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel preparation enriched in rhabdomere membranes to perform a thorough characterization of the lipidomics of Drosophila rhabdomeres. Isolated eyes (500) were homogenized and subjected to a differential centrifugation protocol that generates a fraction enriched in rhabdomere membrane. Lipids extracted from this preparation were identified and quantified by gas chromatography coupled to mass spectrometry. We found an abundance of low sterol esters (C16:0, C18:0), highly abundant and diverse triglycerides, free fatty acids, a moderate variety of mono and diacyglycerols (C:16:0, 18:0, C18:1) and abundant phospholipids (principally C18:2). This preparation opens a new avenue for investigating essential aspects of phototransduction.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.3 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010en10.4067/S0716-97602013000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rhabdomere Drosophila microvilli phototransduction TRP channel fatty acid analysis
spellingShingle	Rhabdomere Drosophila microvilli phototransduction TRP channel fatty acid analysis Muñoz,Yorka Fuenzalida,Karen Bronfman,Miguel Gatica,Arnaldo Sepúlveda,Marcelo Bacigalupo,Juan Roth,Alejandro D Delgado,Ricardo Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
description	Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel preparation enriched in rhabdomere membranes to perform a thorough characterization of the lipidomics of Drosophila rhabdomeres. Isolated eyes (500) were homogenized and subjected to a differential centrifugation protocol that generates a fraction enriched in rhabdomere membrane. Lipids extracted from this preparation were identified and quantified by gas chromatography coupled to mass spectrometry. We found an abundance of low sterol esters (C16:0, C18:0), highly abundant and diverse triglycerides, free fatty acids, a moderate variety of mono and diacyglycerols (C:16:0, 18:0, C18:1) and abundant phospholipids (principally C18:2). This preparation opens a new avenue for investigating essential aspects of phototransduction.
author	Muñoz,Yorka Fuenzalida,Karen Bronfman,Miguel Gatica,Arnaldo Sepúlveda,Marcelo Bacigalupo,Juan Roth,Alejandro D Delgado,Ricardo
author_facet	Muñoz,Yorka Fuenzalida,Karen Bronfman,Miguel Gatica,Arnaldo Sepúlveda,Marcelo Bacigalupo,Juan Roth,Alejandro D Delgado,Ricardo
author_sort	Muñoz,Yorka
title	Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
title_short	Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
title_full	Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
title_fullStr	Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
title_full_unstemmed	Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
title_sort	fatty acid composition of drosophila photoreceptor light-sensitive microvilli
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010
work_keys_str_mv	AT munozyorka fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT fuenzalidakaren fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT bronfmanmiguel fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT gaticaarnaldo fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT sepulvedamarcelo fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT bacigalupojuan fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT rothalejandrod fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli AT delgadoricardo fattyacidcompositionofdrosophilaphotoreceptorlightsensitivemicrovilli
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Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli

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