Microdesiccates produced from normal human tears display four distinctive morphological components

Desiccation of human tears on glass surfaces results in fern-like crystalloids. This phenomenon has been associated with tear normality (Tear Ferning Test, TFT) and is used as a diagnostic aid to evaluate patients with Dry-Eye disease. However, TFT is focused on the assessment of only a minor fracti...

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Autores principales:	López Solís,Remigio, Traipe Castro,Leonidas, Salinas Toro,Daniela, Srur,Miguel, Toledo Araya,Héctor
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	Tear Tear ferning test morphometry dark-field microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300012
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spelling	oai:scielo:S0716-976020130003000122014-01-20Microdesiccates produced from normal human tears display four distinctive morphological componentsLópez Solís,RemigioTraipe Castro,LeonidasSalinas Toro,DanielaSrur,MiguelToledo Araya,Héctor Tear Tear ferning test morphometry dark-field microscopy Desiccation of human tears on glass surfaces results in fern-like crystalloids. This phenomenon has been associated with tear normality (Tear Ferning Test, TFT) and is used as a diagnostic aid to evaluate patients with Dry-Eye disease. However, TFT is focused on the assessment of only a minor fraction of desiccated tear samples and considers only the relative abundance and density of fern-like crystalloids. The aim of this study was to characterize morphologically entire desiccated micro volumes of tears from healthy donors. Tear samples were collected from 23 healthy young adult volunteers. Tear aliquots (1-3 μL) were allowed to dry on glass surfaces under ambient conditions of temperature (15-25°C) and relative humidity (40-45%). Dry samples were analyzed by dark-field microscopy. Morphometric data were acquired with Image J software. Tear volume was positively correlated with both area and time of desiccation. Morphological features of multiple microdesiccates produced from a single subject displayed striking similarities whereas tear microdesiccates from different healthy subjects displayed consistent differences but shared a common general design. This design may be mostly represented by the occurrence of four distinctive zones, named as zones I, II, III and Transition band. The main features of these zones are described.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.3 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300012en10.4067/S0716-97602013000300012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Tear Tear ferning test morphometry dark-field microscopy
spellingShingle	Tear Tear ferning test morphometry dark-field microscopy López Solís,Remigio Traipe Castro,Leonidas Salinas Toro,Daniela Srur,Miguel Toledo Araya,Héctor Microdesiccates produced from normal human tears display four distinctive morphological components
description	Desiccation of human tears on glass surfaces results in fern-like crystalloids. This phenomenon has been associated with tear normality (Tear Ferning Test, TFT) and is used as a diagnostic aid to evaluate patients with Dry-Eye disease. However, TFT is focused on the assessment of only a minor fraction of desiccated tear samples and considers only the relative abundance and density of fern-like crystalloids. The aim of this study was to characterize morphologically entire desiccated micro volumes of tears from healthy donors. Tear samples were collected from 23 healthy young adult volunteers. Tear aliquots (1-3 μL) were allowed to dry on glass surfaces under ambient conditions of temperature (15-25°C) and relative humidity (40-45%). Dry samples were analyzed by dark-field microscopy. Morphometric data were acquired with Image J software. Tear volume was positively correlated with both area and time of desiccation. Morphological features of multiple microdesiccates produced from a single subject displayed striking similarities whereas tear microdesiccates from different healthy subjects displayed consistent differences but shared a common general design. This design may be mostly represented by the occurrence of four distinctive zones, named as zones I, II, III and Transition band. The main features of these zones are described.
author	López Solís,Remigio Traipe Castro,Leonidas Salinas Toro,Daniela Srur,Miguel Toledo Araya,Héctor
author_facet	López Solís,Remigio Traipe Castro,Leonidas Salinas Toro,Daniela Srur,Miguel Toledo Araya,Héctor
author_sort	López Solís,Remigio
title	Microdesiccates produced from normal human tears display four distinctive morphological components
title_short	Microdesiccates produced from normal human tears display four distinctive morphological components
title_full	Microdesiccates produced from normal human tears display four distinctive morphological components
title_fullStr	Microdesiccates produced from normal human tears display four distinctive morphological components
title_full_unstemmed	Microdesiccates produced from normal human tears display four distinctive morphological components
title_sort	microdesiccates produced from normal human tears display four distinctive morphological components
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300012
work_keys_str_mv	AT lopezsolisremigio microdesiccatesproducedfromnormalhumantearsdisplayfourdistinctivemorphologicalcomponents AT traipecastroleonidas microdesiccatesproducedfromnormalhumantearsdisplayfourdistinctivemorphologicalcomponents AT salinastorodaniela microdesiccatesproducedfromnormalhumantearsdisplayfourdistinctivemorphologicalcomponents AT srurmiguel microdesiccatesproducedfromnormalhumantearsdisplayfourdistinctivemorphologicalcomponents AT toledoarayahector microdesiccatesproducedfromnormalhumantearsdisplayfourdistinctivemorphologicalcomponents
_version_	1718441504381337600

Microdesiccates produced from normal human tears display four distinctive morphological components

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