A roadmap to directed enzyme evolution and screening systems for biotechnological applications

Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions foun...

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Autores principales:	Martínez,Ronny, Schwaneberg,Ulrich
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	Directed evolution enzyme engineering high throughput screening random mutagenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400011
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spelling	oai:scielo:S0716-976020130004000112014-02-05A roadmap to directed enzyme evolution and screening systems for biotechnological applicationsMartínez,RonnySchwaneberg,Ulrich Directed evolution enzyme engineering high throughput screening random mutagenesis Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specific evolutive pressure. In practice, critical checkpoints in directed evolution are: selection of the starting point, generation of the mutant library, development of the screening assay and analysis of the output of the screening campaign. Each step in directed evolution can be performed using conceptually and technically different approaches, all having inherent advantages and challenges. In this article, we present and discuss in a general overview, challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.4 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400011en10.4067/S0716-97602013000400011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Directed evolution enzyme engineering high throughput screening random mutagenesis
spellingShingle	Directed evolution enzyme engineering high throughput screening random mutagenesis Martínez,Ronny Schwaneberg,Ulrich A roadmap to directed enzyme evolution and screening systems for biotechnological applications
description	Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specific evolutive pressure. In practice, critical checkpoints in directed evolution are: selection of the starting point, generation of the mutant library, development of the screening assay and analysis of the output of the screening campaign. Each step in directed evolution can be performed using conceptually and technically different approaches, all having inherent advantages and challenges. In this article, we present and discuss in a general overview, challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes.
author	Martínez,Ronny Schwaneberg,Ulrich
author_facet	Martínez,Ronny Schwaneberg,Ulrich
author_sort	Martínez,Ronny
title	A roadmap to directed enzyme evolution and screening systems for biotechnological applications
title_short	A roadmap to directed enzyme evolution and screening systems for biotechnological applications
title_full	A roadmap to directed enzyme evolution and screening systems for biotechnological applications
title_fullStr	A roadmap to directed enzyme evolution and screening systems for biotechnological applications
title_full_unstemmed	A roadmap to directed enzyme evolution and screening systems for biotechnological applications
title_sort	roadmap to directed enzyme evolution and screening systems for biotechnological applications
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400011
work_keys_str_mv	AT martinezronny aroadmaptodirectedenzymeevolutionandscreeningsystemsforbiotechnologicalapplications AT schwanebergulrich aroadmaptodirectedenzymeevolutionandscreeningsystemsforbiotechnologicalapplications AT martinezronny roadmaptodirectedenzymeevolutionandscreeningsystemsforbiotechnologicalapplications AT schwanebergulrich roadmaptodirectedenzymeevolutionandscreeningsystemsforbiotechnologicalapplications
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A roadmap to directed enzyme evolution and screening systems for biotechnological applications

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