Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos

Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental pote...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Arias,María E, Ross,Pablo J, Felmer,Ricardo N
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	Cattle gene expression KSOM nuclear transfer embryos SOF
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400016
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602013000400016
record_format	dspace
spelling	oai:scielo:S0716-976020130004000162014-03-05Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryosArias,María ERoss,Pablo JFelmer,Ricardo N Cattle gene expression KSOM nuclear transfer embryos SOF Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental potential and quality of bovine SCNT embryos cultured in different two-step culture media based on KSOM, SOF and the macromolecules FBS and BSA (K-K/FBS, K-S/BSA and K-K/BSA, respectively). No differences were observed in the cleavage rate for any of the culture systems. However, there was a significant difference (P<0.01) in the rate of blastocyst development, with the K-K/ FBS culture system yielding a higher rate of blastocysts (28%) compared to other treatments (18 and 15%, for K-S/BSA and K-K/BSA, respectively). Although quality of embryos, as assessed by the total number of cells, was not different, the apoptosis index was significantly affected in the sequential culture system (K-S/BSA). Gene expression analysis showed alterations of DNMT1, IGF2, LIF, and PRDX6 genes in embryos cultured in K-S/FBS and of SOD2 in embryos cultured in K-K/BSA. In conclusion, we demonstrated that culture medium may affect not only the developmental potential of SCNT embryos but also, more importantly, the gene expression pattern and apoptotic index, presenting the possibility to manipulate the culture medium composition to modulate global gene expression and improve the overall efficiency of this technique.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.46 n.4 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400016en10.4067/S0716-97602013000400016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cattle gene expression KSOM nuclear transfer embryos SOF
spellingShingle	Cattle gene expression KSOM nuclear transfer embryos SOF Arias,María E Ross,Pablo J Felmer,Ricardo N Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
description	Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental potential and quality of bovine SCNT embryos cultured in different two-step culture media based on KSOM, SOF and the macromolecules FBS and BSA (K-K/FBS, K-S/BSA and K-K/BSA, respectively). No differences were observed in the cleavage rate for any of the culture systems. However, there was a significant difference (P<0.01) in the rate of blastocyst development, with the K-K/ FBS culture system yielding a higher rate of blastocysts (28%) compared to other treatments (18 and 15%, for K-S/BSA and K-K/BSA, respectively). Although quality of embryos, as assessed by the total number of cells, was not different, the apoptosis index was significantly affected in the sequential culture system (K-S/BSA). Gene expression analysis showed alterations of DNMT1, IGF2, LIF, and PRDX6 genes in embryos cultured in K-S/FBS and of SOD2 in embryos cultured in K-K/BSA. In conclusion, we demonstrated that culture medium may affect not only the developmental potential of SCNT embryos but also, more importantly, the gene expression pattern and apoptotic index, presenting the possibility to manipulate the culture medium composition to modulate global gene expression and improve the overall efficiency of this technique.
author	Arias,María E Ross,Pablo J Felmer,Ricardo N
author_facet	Arias,María E Ross,Pablo J Felmer,Ricardo N
author_sort	Arias,María E
title	Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
title_short	Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
title_full	Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
title_fullStr	Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
title_full_unstemmed	Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
title_sort	culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (scnt) embryos
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400016
work_keys_str_mv	AT ariasmariae culturemediumcompositionaffectsthegeneexpressionpatternandinvitrodevelopmentpotentialofbovinesomaticcellnucleartransferscntembryos AT rosspabloj culturemediumcompositionaffectsthegeneexpressionpatternandinvitrodevelopmentpotentialofbovinesomaticcellnucleartransferscntembryos AT felmerricardon culturemediumcompositionaffectsthegeneexpressionpatternandinvitrodevelopmentpotentialofbovinesomaticcellnucleartransferscntembryos
_version_	1718441509312790528

Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos

Ejemplares similares