Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes

BACKGROUND: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains from different bivalents. The meiotic nuclear architecture depends on the chromosome characteristics and consequently is prone to modification by...

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Autores principales:	Berríos,Soledad, Manieu,Catalina, López-Fenner,Julio, Ayarza,Eliana, Page,Jesús, González,Marisel, Manterola,Marcia, Fernández-Donoso,Raúl
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Robertsonian chromosomes Mouse spermatocytes Bivalent associations Nuclear architecture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100016
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spelling	oai:scielo:S0716-976020140001000162015-10-30Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytesBerríos,SoledadManieu,CatalinaLópez-Fenner,JulioAyarza,ElianaPage,JesúsGonzález,MariselManterola,MarciaFernández-Donoso,Raúl Robertsonian chromosomes Mouse spermatocytes Bivalent associations Nuclear architecture BACKGROUND: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains from different bivalents. The meiotic nuclear architecture depends on the chromosome characteristics and consequently is prone to modification by chromosomal rearrangements. In this work, we consider Mus domesticus spermatocytes with diploid chromosome number 2n = 40, all telocentric, and investigate a possible modification of the ancestral nuclear architecture due to the emergence of derived Rb chromosomes, which may be present in the homozygous or heterozygous condition. RESULTS: In the 2n = 40 spermatocyte nuclei random associations mediated by pericentromeric heterochromatin among the 19 telocentric bivalents ocurr at the nuclear periphery. The observed frequency of associations among them, made distinguishable by specific probes and FISH, seems to be the same for pairs that may or may not form Rb chromosomes. In the homozygote Rb 2n = 24 spermatocytes, associations also mediated by pericentromeric heterochromatin occur mainly between the three telocentric or the eight metacentric bivalents themselves. In heterozygote Rb 2n = 32 spermatocytes all heterochromatin is localized at the nuclear periphery, yet associations are mainly observed among the three telocentric bivalents and between the asynaptic axes of the trivalents. CONCLUSIONS: The Rb chromosomes pose sharp restrictions for interactions in the 2n = 24 and 2n = 32 spermatocytes, as compared to the ample possibilities for interactions between bivalents in the 2n = 40 spermatocytes. Undoubtedly the emergence of Rb chromosomes changes the ancestral nuclear architecture of 2n = 40 spermatocytes since they establish new types of interactions among chromosomal domains, particularly through centromeric and heterochromatic regions at the nuclear periphery among telocentric and at the nuclear center among Rb metacentric ones.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100016en10.1186/0717-6287-47-16
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Robertsonian chromosomes Mouse spermatocytes Bivalent associations Nuclear architecture
spellingShingle	Robertsonian chromosomes Mouse spermatocytes Bivalent associations Nuclear architecture Berríos,Soledad Manieu,Catalina López-Fenner,Julio Ayarza,Eliana Page,Jesús González,Marisel Manterola,Marcia Fernández-Donoso,Raúl Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
description	BACKGROUND: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains from different bivalents. The meiotic nuclear architecture depends on the chromosome characteristics and consequently is prone to modification by chromosomal rearrangements. In this work, we consider Mus domesticus spermatocytes with diploid chromosome number 2n = 40, all telocentric, and investigate a possible modification of the ancestral nuclear architecture due to the emergence of derived Rb chromosomes, which may be present in the homozygous or heterozygous condition. RESULTS: In the 2n = 40 spermatocyte nuclei random associations mediated by pericentromeric heterochromatin among the 19 telocentric bivalents ocurr at the nuclear periphery. The observed frequency of associations among them, made distinguishable by specific probes and FISH, seems to be the same for pairs that may or may not form Rb chromosomes. In the homozygote Rb 2n = 24 spermatocytes, associations also mediated by pericentromeric heterochromatin occur mainly between the three telocentric or the eight metacentric bivalents themselves. In heterozygote Rb 2n = 32 spermatocytes all heterochromatin is localized at the nuclear periphery, yet associations are mainly observed among the three telocentric bivalents and between the asynaptic axes of the trivalents. CONCLUSIONS: The Rb chromosomes pose sharp restrictions for interactions in the 2n = 24 and 2n = 32 spermatocytes, as compared to the ample possibilities for interactions between bivalents in the 2n = 40 spermatocytes. Undoubtedly the emergence of Rb chromosomes changes the ancestral nuclear architecture of 2n = 40 spermatocytes since they establish new types of interactions among chromosomal domains, particularly through centromeric and heterochromatic regions at the nuclear periphery among telocentric and at the nuclear center among Rb metacentric ones.
author	Berríos,Soledad Manieu,Catalina López-Fenner,Julio Ayarza,Eliana Page,Jesús González,Marisel Manterola,Marcia Fernández-Donoso,Raúl
author_facet	Berríos,Soledad Manieu,Catalina López-Fenner,Julio Ayarza,Eliana Page,Jesús González,Marisel Manterola,Marcia Fernández-Donoso,Raúl
author_sort	Berríos,Soledad
title	Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
title_short	Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
title_full	Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
title_fullStr	Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
title_full_unstemmed	Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
title_sort	robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100016
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Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes

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