Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity

BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepa...

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Autores principales:	Yan,Yizhong, Li,Jianjun, Zhang,Yiya, Peng,Xiaozhen, Guo,Tianyao, Wang,Jirong, Hu,Weijun, Duan,Zhigui, Wang,Xianchun
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Black widow spider (L. tredecimguttatus) Protein composition Egg Molecular mechanism Property analysis Toxicity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017
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spelling	oai:scielo:S0716-976020140001000172015-10-30Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicityYan,YizhongLi,JianjunZhang,YiyaPeng,XiaozhenGuo,TianyaoWang,JirongHu,WeijunDuan,ZhiguiWang,Xianchun Black widow spider (L. tredecimguttatus) Protein composition Egg Molecular mechanism Property analysis Toxicity BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017en10.1186/0717-6287-47-17
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Black widow spider (L. tredecimguttatus) Protein composition Egg Molecular mechanism Property analysis Toxicity
spellingShingle	Black widow spider (L. tredecimguttatus) Protein composition Egg Molecular mechanism Property analysis Toxicity Yan,Yizhong Li,Jianjun Zhang,Yiya Peng,Xiaozhen Guo,Tianyao Wang,Jirong Hu,Weijun Duan,Zhigui Wang,Xianchun Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
description	BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions.
author	Yan,Yizhong Li,Jianjun Zhang,Yiya Peng,Xiaozhen Guo,Tianyao Wang,Jirong Hu,Weijun Duan,Zhigui Wang,Xianchun
author_facet	Yan,Yizhong Li,Jianjun Zhang,Yiya Peng,Xiaozhen Guo,Tianyao Wang,Jirong Hu,Weijun Duan,Zhigui Wang,Xianchun
author_sort	Yan,Yizhong
title	Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
title_short	Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
title_full	Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
title_fullStr	Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
title_full_unstemmed	Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
title_sort	physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017
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Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity

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