Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment

BACKGROUND: Fern-like crystalloids form when a microvolume of tear is allowed to dry out at ambient conditions on a glass surface. Presence of crystalloids in tear "microdesiccates" is used to evaluate patients with Dry-Eye disease. This study aims to examine morphologically the desiccatio...

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Autores principales:	Traipe-Castro,Leonidas, Salinas-Toro,Daniela, López,Daniela, Zanolli,Mario, Srur,Miguel, Valenzuela,Felipe, Cáceres,Aníbal, Toledo-Araya,Héctor, López-Solís,Remigio
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Tear Tear ferning test Dry eye Dark-field microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100025
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spelling	oai:scielo:S0716-976020140001000252015-10-30Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessmentTraipe-Castro,LeonidasSalinas-Toro,DanielaLópez,DanielaZanolli,MarioSrur,MiguelValenzuela,FelipeCáceres,AníbalToledo-Araya,HéctorLópez-Solís,Remigio Tear Tear ferning test Dry eye Dark-field microscopy BACKGROUND: Fern-like crystalloids form when a microvolume of tear is allowed to dry out at ambient conditions on a glass surface. Presence of crystalloids in tear "microdesiccates" is used to evaluate patients with Dry-Eye disease. This study aims to examine morphologically the desiccation process of normal tear fluid and to identify changes associated with accelerated tear evaporation. Tear microdesiccates from healthy (Non-Dry Eye) and Dry Eye subjects were produced at ambient conditions. Microdesiccate formation was monitored continuously by dark-field video microscopy. Additionally, accelerated desiccation of tear samples from healthy subjects was conducted under controlled experimental conditions. Particular morphological domains of tear microdesiccates and their progressive appearance during desiccation were compared. RESULTS: In normal tear microdesiccates, four distinctive morphological domains (zones I, II, III and transition band) were recognized. Stepwise formation of those domains is now described. Experimentally accelerated desiccation resulted in marked changes in some of those zones, particularly involving either disappearance or size reduction of fern-like crystalloids of zones II and III. Tear microdesiccates from Dry Eye subjects may also display those differences and be the expression of a more synchronous formation of microdesiccate domains. CONCLUSION: Morphological characteristics of tear microdesiccates can provide insights into the relative rate of tear evaporation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100025en10.1186/0717-6287-47-25
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Tear Tear ferning test Dry eye Dark-field microscopy
spellingShingle	Tear Tear ferning test Dry eye Dark-field microscopy Traipe-Castro,Leonidas Salinas-Toro,Daniela López,Daniela Zanolli,Mario Srur,Miguel Valenzuela,Felipe Cáceres,Aníbal Toledo-Araya,Héctor López-Solís,Remigio Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
description	BACKGROUND: Fern-like crystalloids form when a microvolume of tear is allowed to dry out at ambient conditions on a glass surface. Presence of crystalloids in tear "microdesiccates" is used to evaluate patients with Dry-Eye disease. This study aims to examine morphologically the desiccation process of normal tear fluid and to identify changes associated with accelerated tear evaporation. Tear microdesiccates from healthy (Non-Dry Eye) and Dry Eye subjects were produced at ambient conditions. Microdesiccate formation was monitored continuously by dark-field video microscopy. Additionally, accelerated desiccation of tear samples from healthy subjects was conducted under controlled experimental conditions. Particular morphological domains of tear microdesiccates and their progressive appearance during desiccation were compared. RESULTS: In normal tear microdesiccates, four distinctive morphological domains (zones I, II, III and transition band) were recognized. Stepwise formation of those domains is now described. Experimentally accelerated desiccation resulted in marked changes in some of those zones, particularly involving either disappearance or size reduction of fern-like crystalloids of zones II and III. Tear microdesiccates from Dry Eye subjects may also display those differences and be the expression of a more synchronous formation of microdesiccate domains. CONCLUSION: Morphological characteristics of tear microdesiccates can provide insights into the relative rate of tear evaporation.
author	Traipe-Castro,Leonidas Salinas-Toro,Daniela López,Daniela Zanolli,Mario Srur,Miguel Valenzuela,Felipe Cáceres,Aníbal Toledo-Araya,Héctor López-Solís,Remigio
author_facet	Traipe-Castro,Leonidas Salinas-Toro,Daniela López,Daniela Zanolli,Mario Srur,Miguel Valenzuela,Felipe Cáceres,Aníbal Toledo-Araya,Héctor López-Solís,Remigio
author_sort	Traipe-Castro,Leonidas
title	Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
title_short	Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
title_full	Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
title_fullStr	Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
title_full_unstemmed	Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
title_sort	dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100025
work_keys_str_mv	AT traipecastroleonidas dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT salinastorodaniela dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT lopezdaniela dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT zanollimario dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT srurmiguel dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT valenzuelafelipe dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT caceresanibal dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT toledoarayahector dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment AT lopezsolisremigio dynamicsoftearfluiddesiccationonaglasssurfaceacontributiontotearqualityassessment
_version_	1718441515527700480

Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment

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