Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii

BACKGROUND: Termitomyces heimii is a basidiomycete fungus that has a symbiotic relationship with termites, and it is an edible mushroom with a unique flavour and texture. T. heimii is also one of the most difficult mushrooms to cultivate throughout the world. Little is known about the growth and dev...

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Autores principales:	Norasfaliza Rahmad,Jameel R Al-Obaidi, Rashid,Noraswati Mohd Nor, Zean,Ng Boon, Yusoff,Mohd Hafis Yuswan Mohd, Shaharuddin,Nur Syahidah, Jamiland,Nor Azreen Mohd, Saleh,Norihan Mohd
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100030
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spelling	oai:scielo:S0716-976020140001000302015-10-30Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimiiNorasfaliza Rahmad,Jameel R Al-ObaidiRashid,Noraswati Mohd NorZean,Ng BoonYusoff,Mohd Hafis Yuswan MohdShaharuddin,Nur SyahidahJamiland,Nor Azreen MohdSaleh,Norihan MohdBACKGROUND: Termitomyces heimii is a basidiomycete fungus that has a symbiotic relationship with termites, and it is an edible mushroom with a unique flavour and texture. T. heimii is also one of the most difficult mushrooms to cultivate throughout the world. Little is known about the growth and development of these mushrooms, and the available information is insufficient or poor. The purpose of this study was to provide a base of knowledge regarding the biological processes involved in the development of T. heimii. The proteomic method of 2 dimensional difference gel electrophoresis 2D-DIGE was used to determine and examine the protein profiles of each developmental stage (mycelium, primordium and fruiting body). Total proteins were extracted by TCA-acetone precipitation. RESULTS: A total of 271 protein spots were detected by electrophoresis covering pH 3 - 10 and 10 - 250 kDa. Selected protein spots were subjected to mass spectrometric analyses with matrix-assisted laser desorption/ionisation (MALDI TOF/TOF). Nineteen protein spots were identified based on peptide mass fingerprinting by matching peptide fragments to the NCBI non-redundant database using MASCOT software. The 19 protein spots were categorised into four major groups through KEGG pathway analysis, as follows: carbohydrate metabolism, energy metabolism, amino acid metabolism and response to environmental stress. CONCLUSIONS: The results from our study show that there is a clear correlation between the changes in protein expression that occur during different developmental stages. Enzymes related to cell wall synthesis were most highly expressed during fruiting body formation compared to the mycelium and primordial stages. Moreover, enzymes involved in cell wall component degradation were up-regulated in the earlier stages of mushroom development.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100030en10.1186/0717-6287-47-30
institution	Scielo Chile
collection	Scielo Chile
language	English
description	BACKGROUND: Termitomyces heimii is a basidiomycete fungus that has a symbiotic relationship with termites, and it is an edible mushroom with a unique flavour and texture. T. heimii is also one of the most difficult mushrooms to cultivate throughout the world. Little is known about the growth and development of these mushrooms, and the available information is insufficient or poor. The purpose of this study was to provide a base of knowledge regarding the biological processes involved in the development of T. heimii. The proteomic method of 2 dimensional difference gel electrophoresis 2D-DIGE was used to determine and examine the protein profiles of each developmental stage (mycelium, primordium and fruiting body). Total proteins were extracted by TCA-acetone precipitation. RESULTS: A total of 271 protein spots were detected by electrophoresis covering pH 3 - 10 and 10 - 250 kDa. Selected protein spots were subjected to mass spectrometric analyses with matrix-assisted laser desorption/ionisation (MALDI TOF/TOF). Nineteen protein spots were identified based on peptide mass fingerprinting by matching peptide fragments to the NCBI non-redundant database using MASCOT software. The 19 protein spots were categorised into four major groups through KEGG pathway analysis, as follows: carbohydrate metabolism, energy metabolism, amino acid metabolism and response to environmental stress. CONCLUSIONS: The results from our study show that there is a clear correlation between the changes in protein expression that occur during different developmental stages. Enzymes related to cell wall synthesis were most highly expressed during fruiting body formation compared to the mycelium and primordial stages. Moreover, enzymes involved in cell wall component degradation were up-regulated in the earlier stages of mushroom development.
author	Norasfaliza Rahmad,Jameel R Al-Obaidi Rashid,Noraswati Mohd Nor Zean,Ng Boon Yusoff,Mohd Hafis Yuswan Mohd Shaharuddin,Nur Syahidah Jamiland,Nor Azreen Mohd Saleh,Norihan Mohd
spellingShingle	Norasfaliza Rahmad,Jameel R Al-Obaidi Rashid,Noraswati Mohd Nor Zean,Ng Boon Yusoff,Mohd Hafis Yuswan Mohd Shaharuddin,Nur Syahidah Jamiland,Nor Azreen Mohd Saleh,Norihan Mohd Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
author_facet	Norasfaliza Rahmad,Jameel R Al-Obaidi Rashid,Noraswati Mohd Nor Zean,Ng Boon Yusoff,Mohd Hafis Yuswan Mohd Shaharuddin,Nur Syahidah Jamiland,Nor Azreen Mohd Saleh,Norihan Mohd
author_sort	Norasfaliza Rahmad,Jameel R Al-Obaidi
title	Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
title_short	Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
title_full	Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
title_fullStr	Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
title_full_unstemmed	Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
title_sort	comparative proteomic analysis of different developmental stages of the edible mushroom termitomyces heimii
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100030
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Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii

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