Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation

BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in...

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Autores principales: Urriola-Muñoz,Paulina, Lizama,Carlos, Lagos-Cabré,Raúl, Reyes,Juan G, Moreno,Ricardo D
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2014
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100031
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spelling oai:scielo:S0716-976020140001000312015-10-30Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiationUrriola-Muñoz,PaulinaLizama,CarlosLagos-Cabré,RaúlReyes,Juan GMoreno,Ricardo D Testis Spermatogenesis Apoptosis Paracrine Metalloproteinase BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis. CONCLUSIONS: Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100031en10.1186/0717-6287-47-31
institution Scielo Chile
collection Scielo Chile
language English
topic Testis
Spermatogenesis
Apoptosis
Paracrine
Metalloproteinase
spellingShingle Testis
Spermatogenesis
Apoptosis
Paracrine
Metalloproteinase
Urriola-Muñoz,Paulina
Lizama,Carlos
Lagos-Cabré,Raúl
Reyes,Juan G
Moreno,Ricardo D
Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
description BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis. CONCLUSIONS: Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.
author Urriola-Muñoz,Paulina
Lizama,Carlos
Lagos-Cabré,Raúl
Reyes,Juan G
Moreno,Ricardo D
author_facet Urriola-Muñoz,Paulina
Lizama,Carlos
Lagos-Cabré,Raúl
Reyes,Juan G
Moreno,Ricardo D
author_sort Urriola-Muñoz,Paulina
title Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
title_short Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
title_full Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
title_fullStr Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
title_full_unstemmed Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
title_sort differential expression and localization of adam10 and adam17 during rat spermatogenesis suggest a role in germ cell differentiation
publisher Sociedad de Biología de Chile
publishDate 2014
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100031
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AT lizamacarlos differentialexpressionandlocalizationofadam10andadam17duringratspermatogenesissuggestaroleingermcelldifferentiation
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