Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy

BACKGROUND: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them alumin...

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Autores principales:	Matei,Clara, Tampa,Mircea, Caruntu,Constantin, Ion,Rodica-Mariana, Georgescu,Simona-Roxana, Dumitrascu,Georgiana Roxana, Constantin,Carolina, Neagu,Monica
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Aluminum di-sulphonated phthalocyanine DOK cells Photodynamic therapy Protein microarray
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100033
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spelling	oai:scielo:S0716-976020140001000332015-10-30Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapyMatei,ClaraTampa,MirceaCaruntu,ConstantinIon,Rodica-MarianaGeorgescu,Simona-RoxanaDumitrascu,Georgiana RoxanaConstantin,CarolinaNeagu,Monica Aluminum di-sulphonated phthalocyanine DOK cells Photodynamic therapy Protein microarray BACKGROUND: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential. RESULTS: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude. CONCLUSIONS: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100033en10.1186/0717-6287-47-33
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aluminum di-sulphonated phthalocyanine DOK cells Photodynamic therapy Protein microarray
spellingShingle	Aluminum di-sulphonated phthalocyanine DOK cells Photodynamic therapy Protein microarray Matei,Clara Tampa,Mircea Caruntu,Constantin Ion,Rodica-Mariana Georgescu,Simona-Roxana Dumitrascu,Georgiana Roxana Constantin,Carolina Neagu,Monica Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
description	BACKGROUND: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential. RESULTS: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude. CONCLUSIONS: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.
author	Matei,Clara Tampa,Mircea Caruntu,Constantin Ion,Rodica-Mariana Georgescu,Simona-Roxana Dumitrascu,Georgiana Roxana Constantin,Carolina Neagu,Monica
author_facet	Matei,Clara Tampa,Mircea Caruntu,Constantin Ion,Rodica-Mariana Georgescu,Simona-Roxana Dumitrascu,Georgiana Roxana Constantin,Carolina Neagu,Monica
author_sort	Matei,Clara
title	Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
title_short	Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
title_full	Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
title_fullStr	Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
title_full_unstemmed	Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
title_sort	protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100033
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Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy

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