Apoptosis in HEp-2 cells infected with Ureaplasma diversum

BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes ac...

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Autores principales:	Amorim,Aline Teixeira, Marques,Lucas Miranda, Santos,Angelita Maria Oliveira Gusmão, Martins,Hellen Braga, Barbosa,Maysa Santos, Rezende,Izadora Souza, Andrade,Ewerton Ferraz, Campos,Guilherme Barreto, Lobão,Tássia Neves, Cortez,Beatriz Araujo, Monezi,Telma Alvez, Machado-Santelli,Glaucia Maria, Timenetsky,Jorge
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Ureaplasma diversum Invasion Apoptosis HEp-2 cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038
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spelling	oai:scielo:S0716-976020140001000382015-10-30Apoptosis in HEp-2 cells infected with Ureaplasma diversumAmorim,Aline TeixeiraMarques,Lucas MirandaSantos,Angelita Maria Oliveira GusmãoMartins,Hellen BragaBarbosa,Maysa SantosRezende,Izadora SouzaAndrade,Ewerton FerrazCampos,Guilherme BarretoLobão,Tássia NevesCortez,Beatriz AraujoMonezi,Telma AlvezMachado-Santelli,Glaucia MariaTimenetsky,Jorge Ureaplasma diversum Invasion Apoptosis HEp-2 cells BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038en10.1186/0717-6287-47-38
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ureaplasma diversum Invasion Apoptosis HEp-2 cells
spellingShingle	Ureaplasma diversum Invasion Apoptosis HEp-2 cells Amorim,Aline Teixeira Marques,Lucas Miranda Santos,Angelita Maria Oliveira Gusmão Martins,Hellen Braga Barbosa,Maysa Santos Rezende,Izadora Souza Andrade,Ewerton Ferraz Campos,Guilherme Barreto Lobão,Tássia Neves Cortez,Beatriz Araujo Monezi,Telma Alvez Machado-Santelli,Glaucia Maria Timenetsky,Jorge Apoptosis in HEp-2 cells infected with Ureaplasma diversum
description	BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.
author	Amorim,Aline Teixeira Marques,Lucas Miranda Santos,Angelita Maria Oliveira Gusmão Martins,Hellen Braga Barbosa,Maysa Santos Rezende,Izadora Souza Andrade,Ewerton Ferraz Campos,Guilherme Barreto Lobão,Tássia Neves Cortez,Beatriz Araujo Monezi,Telma Alvez Machado-Santelli,Glaucia Maria Timenetsky,Jorge
author_facet	Amorim,Aline Teixeira Marques,Lucas Miranda Santos,Angelita Maria Oliveira Gusmão Martins,Hellen Braga Barbosa,Maysa Santos Rezende,Izadora Souza Andrade,Ewerton Ferraz Campos,Guilherme Barreto Lobão,Tássia Neves Cortez,Beatriz Araujo Monezi,Telma Alvez Machado-Santelli,Glaucia Maria Timenetsky,Jorge
author_sort	Amorim,Aline Teixeira
title	Apoptosis in HEp-2 cells infected with Ureaplasma diversum
title_short	Apoptosis in HEp-2 cells infected with Ureaplasma diversum
title_full	Apoptosis in HEp-2 cells infected with Ureaplasma diversum
title_fullStr	Apoptosis in HEp-2 cells infected with Ureaplasma diversum
title_full_unstemmed	Apoptosis in HEp-2 cells infected with Ureaplasma diversum
title_sort	apoptosis in hep-2 cells infected with ureaplasma diversum
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038
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Apoptosis in HEp-2 cells infected with Ureaplasma diversum

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