Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production

Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production

BACKGROUND: The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinen...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lee,Won-Seok, Lim,Jin-Han, Sung,Myung-Soon, Lee,Eun-Gyeong, Oh,Yoo-Jeong, Yoo,Wan-Hee
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2014
Materias:
Angelica sinensis
COX
IL-1β
MMPs
Rheumatoid arthritis (RA)
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100041
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0716-97602014000100041
record_format dspace
spelling oai:scielo:S0716-976020140001000412015-10-30Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs productionLee,Won-SeokLim,Jin-HanSung,Myung-SoonLee,Eun-GyeongOh,Yoo-JeongYoo,Wan-Hee Angelica sinensis COX IL-1β MMPs Rheumatoid arthritis (RA) BACKGROUND: The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinensis (EAAS) on the interleukin-1β (IL-1β)-induced proliferation of rheumatoid arthritis synovial fibroblasts (RASFs), and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) 2, and prostaglandin E2 (PGE2), involved in articular bone and cartilage destruction, by RASFs. RESULTS: RASF proliferation was evaluated with cholecystokinin octapeptide (CCK-8) reagent in the presence of IL-1β with/without EAAS. Expression of MMPs, tissue inhibitor of metalloproteinases-1 (TIMP-1), COXs, PGE2, and intracellular mitogen-activated protein kinase (MAPK) signaling molecules, including p-ERK, p-p38, p-JNK, and NF-κB, were examined using immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. EAAS inhibited IL-1β-induced RASF proliferation; MMP-1, MMP-3, and COX-2 mRNA and protein expressions; and PGE2 production. EAAS also inhibits the phosphorylation of ERK-1/2, p38, and JNK, and activation of NF-κB by IL-1β. CONCLUSION: EAAS might be a new therapeutic modality for rheumatoid arthritis management.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100041en10.1186/0717-6287-47-41
institution Scielo Chile
collection Scielo Chile
language English
topic Angelica sinensis
COX
IL-1β
MMPs
Rheumatoid arthritis (RA)
spellingShingle Angelica sinensis
COX
IL-1β
MMPs
Rheumatoid arthritis (RA)
Lee,Won-Seok
Lim,Jin-Han
Sung,Myung-Soon
Lee,Eun-Gyeong
Oh,Yoo-Jeong
Yoo,Wan-Hee
Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
description BACKGROUND: The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinensis (EAAS) on the interleukin-1β (IL-1β)-induced proliferation of rheumatoid arthritis synovial fibroblasts (RASFs), and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) 2, and prostaglandin E2 (PGE2), involved in articular bone and cartilage destruction, by RASFs. RESULTS: RASF proliferation was evaluated with cholecystokinin octapeptide (CCK-8) reagent in the presence of IL-1β with/without EAAS. Expression of MMPs, tissue inhibitor of metalloproteinases-1 (TIMP-1), COXs, PGE2, and intracellular mitogen-activated protein kinase (MAPK) signaling molecules, including p-ERK, p-p38, p-JNK, and NF-κB, were examined using immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. EAAS inhibited IL-1β-induced RASF proliferation; MMP-1, MMP-3, and COX-2 mRNA and protein expressions; and PGE2 production. EAAS also inhibits the phosphorylation of ERK-1/2, p38, and JNK, and activation of NF-κB by IL-1β. CONCLUSION: EAAS might be a new therapeutic modality for rheumatoid arthritis management.
author Lee,Won-Seok
Lim,Jin-Han
Sung,Myung-Soon
Lee,Eun-Gyeong
Oh,Yoo-Jeong
Yoo,Wan-Hee
author_facet Lee,Won-Seok
Lim,Jin-Han
Sung,Myung-Soon
Lee,Eun-Gyeong
Oh,Yoo-Jeong
Yoo,Wan-Hee
author_sort Lee,Won-Seok
title Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
title_short Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
title_full Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
title_fullStr Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
title_full_unstemmed Ethyl acetate fraction from Angelica sinensis inhibits IL-1β-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
title_sort ethyl acetate fraction from angelica sinensis inhibits il-1β-induced rheumatoid synovial fibroblast proliferation and cox-2, pge2, and mmps production
publisher Sociedad de Biología de Chile
publishDate 2014
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100041
work_keys_str_mv AT leewonseok ethylacetatefractionfromangelicasinensisinhibitsil1946inducedrheumatoidsynovialfibroblastproliferationandcox2pge2andmmpsproduction
AT limjinhan ethylacetatefractionfromangelicasinensisinhibitsil1946inducedrheumatoidsynovialfibroblastproliferationandcox2pge2andmmpsproduction
AT sungmyungsoon ethylacetatefractionfromangelicasinensisinhibitsil1946inducedrheumatoidsynovialfibroblastproliferationandcox2pge2andmmpsproduction
AT leeeungyeong ethylacetatefractionfromangelicasinensisinhibitsil1946inducedrheumatoidsynovialfibroblastproliferationandcox2pge2andmmpsproduction
AT ohyoojeong ethylacetatefractionfromangelicasinensisinhibitsil1946inducedrheumatoidsynovialfibroblastproliferationandcox2pge2andmmpsproduction
AT yoowanhee ethylacetatefractionfromangelicasinensisinhibitsil1946inducedrheumatoidsynovialfibroblastproliferationandcox2pge2andmmpsproduction
_version_ 1718441519716761600

Ejemplares similares