Effect of exogenous nitric oxide on sperm motility in vitro

BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentratio...

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Autores principales:	Wang,Jiangtao, He,Qingliu, Yan,Xingyu, Cai,Youmei, Chen,Junyi
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Nitric oxide Sperm capacitation Sperm motility Time Human
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044
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spelling	oai:scielo:S0716-976020140001000442015-10-30Effect of exogenous nitric oxide on sperm motility in vitroWang,JiangtaoHe,QingliuYan,XingyuCai,YoumeiChen,Junyi Nitric oxide Sperm capacitation Sperm motility Time Human BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of these concentrations using sodium nitroprusside (an NO donor) and NG-monomethyl-L-argenine (an NO synthase inhibitor). RESULTS: Compared to the normal sperm group, the abnormal sperm group had a longer basal time to reach the appropriate concentration of NO (p < 0.001), and the duration of time at this concentration was longer for the abnormal sperm group (p < 0.001). Both the basal time and the duration of time were significantly correlated with sperm viability and percentage of progressive sperm (p < 0.001). The experimental group had a significantly higher percentage of progressive sperm than the control group (p < 0.001). CONCLUSIONS: We hypothesize that there is a certain regularity to both NO concentration and its duration of time in regards to sperm capacitation, and that an adequate duration of time at the appropriate NO concentration is beneficial to sperm motility.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044en10.1186/0717-6287-47-44
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nitric oxide Sperm capacitation Sperm motility Time Human
spellingShingle	Nitric oxide Sperm capacitation Sperm motility Time Human Wang,Jiangtao He,Qingliu Yan,Xingyu Cai,Youmei Chen,Junyi Effect of exogenous nitric oxide on sperm motility in vitro
description	BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of these concentrations using sodium nitroprusside (an NO donor) and NG-monomethyl-L-argenine (an NO synthase inhibitor). RESULTS: Compared to the normal sperm group, the abnormal sperm group had a longer basal time to reach the appropriate concentration of NO (p < 0.001), and the duration of time at this concentration was longer for the abnormal sperm group (p < 0.001). Both the basal time and the duration of time were significantly correlated with sperm viability and percentage of progressive sperm (p < 0.001). The experimental group had a significantly higher percentage of progressive sperm than the control group (p < 0.001). CONCLUSIONS: We hypothesize that there is a certain regularity to both NO concentration and its duration of time in regards to sperm capacitation, and that an adequate duration of time at the appropriate NO concentration is beneficial to sperm motility.
author	Wang,Jiangtao He,Qingliu Yan,Xingyu Cai,Youmei Chen,Junyi
author_facet	Wang,Jiangtao He,Qingliu Yan,Xingyu Cai,Youmei Chen,Junyi
author_sort	Wang,Jiangtao
title	Effect of exogenous nitric oxide on sperm motility in vitro
title_short	Effect of exogenous nitric oxide on sperm motility in vitro
title_full	Effect of exogenous nitric oxide on sperm motility in vitro
title_fullStr	Effect of exogenous nitric oxide on sperm motility in vitro
title_full_unstemmed	Effect of exogenous nitric oxide on sperm motility in vitro
title_sort	effect of exogenous nitric oxide on sperm motility in vitro
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044
work_keys_str_mv	AT wangjiangtao effectofexogenousnitricoxideonspermmotilityinvitro AT heqingliu effectofexogenousnitricoxideonspermmotilityinvitro AT yanxingyu effectofexogenousnitricoxideonspermmotilityinvitro AT caiyoumei effectofexogenousnitricoxideonspermmotilityinvitro AT chenjunyi effectofexogenousnitricoxideonspermmotilityinvitro
_version_	1718441520400433152

Effect of exogenous nitric oxide on sperm motility in vitro

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